TY - JOUR
T1 - An assessment of the toxicity of metals to Pseudomonas aeruginosa PU21 (Rip64)
AU - Chen, Bor Yann
AU - Wu, Chih Hui
AU - Chang, Jo Shu
N1 - Funding Information:
Financial support (NSC 91-2214-E-197-001, NSC 92-2214-E-197-001, and NSC 93-2214-E-197-002) from National Science Council, Taiwan, ROC for this research and seeding grants for Biochemical Engineering Laboratory of National I-Lan University from the Ministry of Education, Taiwan, ROC are very much appreciated. Part of crucial results were presented in Proceedings of the 4th International Conference on Remediation of Chlorinated and Recalcitrant Compounds (Monterey, CA; May 2004), Paper 4C-25, in: A.R. Gavaskar and A.S.C. Chen (Eds.), Remediation of Chlorinated and Recalcitrant Compounds—2004. Most of innovative concepts were initiated while the first author (B.-Y. Chen) held a research associate awardship from National Research Council (NRC), USA to work in National Risk Management Research Laboratory (NRMRL), US Environmental Protection Agency, Cincinnati, OH, USA. The authors also sincerely appreciate comments of great importance from anonymous reviewers.
PY - 2006/10
Y1 - 2006/10
N2 - The toxicity of Co(II), Mn(II), Cd(II), and Zn(II) for Pseudomonas aeruginosa PU21, a Hg(II)-hyperresistant strain containing the mercury resistance mer operon, was determined. The metal tolerance of PU21 was strongly influenced by environmental conditions (e.g., existing metal, medium composition). Dose-response analysis on chronic and acute toxicity (e.g., EC20, median effective dose EC50, and slope factor B) of divalent cobalt, manganese, cadmium, and zinc cations in LB medium amended with citric acid phosphate buffered saline (CAPBS) suggested a toxicity series of Co > Mn ≈ Zn > Cd for EC50. In contrast, excluding the likely precipitate of Zn(II), the toxicity ranking in phosphate-buffered saline (PBS)-amended LB medium was Co > Cd > Mn. The metal toxicity in PBS, irrespective of metals, was greater than that in CAPBS. This might be attributed to the presence of citric acid in CAPBS as a chelating ligand donating electrons to hold free metals (e.g., Cd2+, Zn2+ tetrahedral ML4 complex). The toxicity assessment established viable operation ranges (ca. <EC50-EC70) to prevent operation failures for metal detoxification and biosorption of mine wastewater treatment by P. aeruginosa PU21.
AB - The toxicity of Co(II), Mn(II), Cd(II), and Zn(II) for Pseudomonas aeruginosa PU21, a Hg(II)-hyperresistant strain containing the mercury resistance mer operon, was determined. The metal tolerance of PU21 was strongly influenced by environmental conditions (e.g., existing metal, medium composition). Dose-response analysis on chronic and acute toxicity (e.g., EC20, median effective dose EC50, and slope factor B) of divalent cobalt, manganese, cadmium, and zinc cations in LB medium amended with citric acid phosphate buffered saline (CAPBS) suggested a toxicity series of Co > Mn ≈ Zn > Cd for EC50. In contrast, excluding the likely precipitate of Zn(II), the toxicity ranking in phosphate-buffered saline (PBS)-amended LB medium was Co > Cd > Mn. The metal toxicity in PBS, irrespective of metals, was greater than that in CAPBS. This might be attributed to the presence of citric acid in CAPBS as a chelating ligand donating electrons to hold free metals (e.g., Cd2+, Zn2+ tetrahedral ML4 complex). The toxicity assessment established viable operation ranges (ca. <EC50-EC70) to prevent operation failures for metal detoxification and biosorption of mine wastewater treatment by P. aeruginosa PU21.
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U2 - 10.1016/j.biortech.2005.08.022
DO - 10.1016/j.biortech.2005.08.022
M3 - Article
C2 - 16243523
AN - SCOPUS:33745206027
VL - 97
SP - 1880
EP - 1886
JO - Agricultural Wastes
JF - Agricultural Wastes
SN - 0960-8524
IS - 15
ER -