An assessment of the toxicity of metals to Pseudomonas aeruginosa PU21 (Rip64)

Bor Yann Chen, Chih Hui Wu, Jo Shu Chang

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24 Citations (Scopus)

Abstract

The toxicity of Co(II), Mn(II), Cd(II), and Zn(II) for Pseudomonas aeruginosa PU21, a Hg(II)-hyperresistant strain containing the mercury resistance mer operon, was determined. The metal tolerance of PU21 was strongly influenced by environmental conditions (e.g., existing metal, medium composition). Dose-response analysis on chronic and acute toxicity (e.g., EC20, median effective dose EC50, and slope factor B) of divalent cobalt, manganese, cadmium, and zinc cations in LB medium amended with citric acid phosphate buffered saline (CAPBS) suggested a toxicity series of Co > Mn ≈ Zn > Cd for EC50. In contrast, excluding the likely precipitate of Zn(II), the toxicity ranking in phosphate-buffered saline (PBS)-amended LB medium was Co > Cd > Mn. The metal toxicity in PBS, irrespective of metals, was greater than that in CAPBS. This might be attributed to the presence of citric acid in CAPBS as a chelating ligand donating electrons to hold free metals (e.g., Cd2+, Zn2+ tetrahedral ML4 complex). The toxicity assessment established viable operation ranges (ca. <EC50-EC70) to prevent operation failures for metal detoxification and biosorption of mine wastewater treatment by P. aeruginosa PU21.

Original languageEnglish
Pages (from-to)1880-1886
Number of pages7
JournalBioresource technology
Volume97
Issue number15
DOIs
Publication statusPublished - 2006 Oct 1

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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