An automated microfluidic-based immunoassay cartridge for allergen screening and other multiplexed assays

Lung Wen Tai, Ko Yuan Tseng, Shaw Tsu Wang, Chao Cheng Chiu, Chak Hone Kow, Ping Chang, Chichen Chen, Jiu-Yao Wang, James R. Webster

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A microfluidic cartridge and system for multiplexed immunoassays is described. The passive microfluidic cartridge was composed of three layers of injection molded plastic sealed together using a thermal staking technique. Using this platform technology, a specific immunoglobulin E (IgE) panel assay was constructed. Allergen extract targets, positive and negative controls, and IgE calibration standards were immobilized within the cartridge as a microarray. A computer-controlled solenoid array provided the necessary actuation force for pumping reagents within the cartridge to perform an automated, chemiluminescent indirect immunoassay. A 20-target allergen extract panel was demonstrated on the device with a total analysis time of 27 min. Allergen screening results showed 84% agreement for 3 house dust mites (N = 300) compared with a commercial test and 80% agreement overall (N = 978). Average coefficients of variation (N = 80) were measured as 20.5% for low/medium levels and 20.4% for medium/high levels. The average limit of detection (N = 160) was measured at 0.535 AU, and cutoff levels of 1.0 AU were estimated at less than 1 IU/ml (2.4 ng/ml). Such a system has potential applications in decentralized allergen screening as well as in other near-patient diagnostic immunoassays where multiplexed analysis, ease of use, and short analysis time are critical.

Original languageEnglish
Pages (from-to)98-105
Number of pages8
JournalAnalytical Biochemistry
Volume391
Issue number2
DOIs
Publication statusPublished - 2009 Aug 15

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Microfluidics
Immunoassay
Allergens
Assays
Screening
Immunoglobulin E
Pyroglyphidae
Solenoids
Microarrays
Calibration
Plastics
Dust
Limit of Detection
Hot Temperature
Technology
Equipment and Supplies
Injections

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Tai, L. W., Tseng, K. Y., Wang, S. T., Chiu, C. C., Kow, C. H., Chang, P., ... Webster, J. R. (2009). An automated microfluidic-based immunoassay cartridge for allergen screening and other multiplexed assays. Analytical Biochemistry, 391(2), 98-105. https://doi.org/10.1016/j.ab.2009.05.009
Tai, Lung Wen ; Tseng, Ko Yuan ; Wang, Shaw Tsu ; Chiu, Chao Cheng ; Kow, Chak Hone ; Chang, Ping ; Chen, Chichen ; Wang, Jiu-Yao ; Webster, James R. / An automated microfluidic-based immunoassay cartridge for allergen screening and other multiplexed assays. In: Analytical Biochemistry. 2009 ; Vol. 391, No. 2. pp. 98-105.
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Tai, LW, Tseng, KY, Wang, ST, Chiu, CC, Kow, CH, Chang, P, Chen, C, Wang, J-Y & Webster, JR 2009, 'An automated microfluidic-based immunoassay cartridge for allergen screening and other multiplexed assays', Analytical Biochemistry, vol. 391, no. 2, pp. 98-105. https://doi.org/10.1016/j.ab.2009.05.009

An automated microfluidic-based immunoassay cartridge for allergen screening and other multiplexed assays. / Tai, Lung Wen; Tseng, Ko Yuan; Wang, Shaw Tsu; Chiu, Chao Cheng; Kow, Chak Hone; Chang, Ping; Chen, Chichen; Wang, Jiu-Yao; Webster, James R.

In: Analytical Biochemistry, Vol. 391, No. 2, 15.08.2009, p. 98-105.

Research output: Contribution to journalArticle

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AU - Chang, Ping

AU - Chen, Chichen

AU - Wang, Jiu-Yao

AU - Webster, James R.

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