An automated microfluidic-based immunoassay cartridge for allergen screening and other multiplexed assays

Lung Wen Tai, Ko Yuan Tseng, Shaw Tsu Wang, Chao Cheng Chiu, Chak Hone Kow, Ping Chang, Chichen Chen, Jiu Yao Wang, James R. Webster

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

A microfluidic cartridge and system for multiplexed immunoassays is described. The passive microfluidic cartridge was composed of three layers of injection molded plastic sealed together using a thermal staking technique. Using this platform technology, a specific immunoglobulin E (IgE) panel assay was constructed. Allergen extract targets, positive and negative controls, and IgE calibration standards were immobilized within the cartridge as a microarray. A computer-controlled solenoid array provided the necessary actuation force for pumping reagents within the cartridge to perform an automated, chemiluminescent indirect immunoassay. A 20-target allergen extract panel was demonstrated on the device with a total analysis time of 27 min. Allergen screening results showed 84% agreement for 3 house dust mites (N = 300) compared with a commercial test and 80% agreement overall (N = 978). Average coefficients of variation (N = 80) were measured as 20.5% for low/medium levels and 20.4% for medium/high levels. The average limit of detection (N = 160) was measured at 0.535 AU, and cutoff levels of 1.0 AU were estimated at less than 1 IU/ml (2.4 ng/ml). Such a system has potential applications in decentralized allergen screening as well as in other near-patient diagnostic immunoassays where multiplexed analysis, ease of use, and short analysis time are critical.

Original languageEnglish
Pages (from-to)98-105
Number of pages8
JournalAnalytical Biochemistry
Volume391
Issue number2
DOIs
Publication statusPublished - 2009 Aug 15

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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