An automatic microfluidic system for continuous selection of aptamers targeting surface protein by using cancer tissue samples

Wei Ting Liu, Yi Cheng Tsai, Wen Bin Lee, Chien Yu Fu, Yuan Jhe Chuang, Keng Fu Hsu, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Aptamers have been regarded as promising artificial antibodies with high stability. To successfully select aptamers that were able to be directedly adopted for clinical applications, we have developed an integrated microfluidic chip to perform a tissue-based SELEX (tissue-SELEX) in which time and processes of SELEX were reduced and simplified. However, our previous design required a large number of tissue specimen and chips fabricated for individual rounds of SELEX. Moreover, many aptamers targeting cytosolic or nucleus proteins were selected through tissue sections that could not be used in membrane-specific target therapy and diagnosis. In this work, we reported the first automatic microfluidic system which was capable of performing "continuous" SELEX with clinical "tissue" samples, which only took 12 hours to complete 6 rounds of SELEX with minimum volume of tissue samples and reagents. In addition, in order to further improve the selection outcome, a single-stranded DNA (ssDNA) library specific to the cell membrane was pre-selected such that the screened aptamers could be specifically bounded on the cell surfaces for targeting and diagnostic applications.

Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages1910-1913
Number of pages4
ISBN (Electronic)9781510897571
Publication statusPublished - 2018 Jan 1
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan
Duration: 2018 Nov 112018 Nov 15

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume4

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
CountryTaiwan
CityKaohsiung
Period18-11-1118-11-15

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

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