An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration

V. Chia Hsuan Tseng, Chee Ho Chew, Wan Ting Huang, Yang Kao Wang, Ko Shao Chen, Szu Yi Chou, Chien Chung Chen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Recently, a novel substrate known as an electrospun polylactic acid (PLLA) microtube array membrane (MTAM) was successfully developed as a cell coculture platform. Structurally, this substrate is made up of one-to-one connected, ultrathin, submicron scale fibers that are arranged in an arrayed formation. Its unique structure confers several key advantages which are beneficial in a cell coculture system. In this study, the interaction between rat fetal neural stem cells (NSC) and astrocytes was examined by comparing the outcome of a typical Transwell-based coculture system and that of an electrospun PLLA MTAM-based coculture system. Compared to tissue culture polystyrene (TCP) and Transwell coculture inserts, a superior cell viability of NSC was observed when cultured in lumens of electrospun PLLA MTAM (with supportive immunostaining images). Reverse transcription polymerase chain reaction revealed a strong interaction between astrocytes and NSC through a higher expression of doublecortin and a lower expression of nestin. These data demonstrate that MTAM is clearly a better coculture platform than the traditional Transwell system.

Original languageEnglish
Pages (from-to)179-190
Number of pages12
JournalCells Tissues Organs
Volume204
Issue number3-4
DOIs
Publication statusPublished - 2017 Oct 1

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All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology

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