An electro-microchip for immunoassay using gold nanoparticle and silver enhancement

Keng Shiang Huang; Hao Hsuan Huang; Tsung Chain Chang; Hong Ping Lin; Yung Chiang Chung; Yu Cheng Lin

An electro-microchip for immunoassay using gold nanoparticle and silver enhancement

Keng Shiang Huang, Hao Hsuan Huang, Tsung Chain Chang, Hong Ping Lin, Yung Chiang Chung, Yu Cheng Lin

Research output: Contribution to conference › Paper › peer-review

Abstract

This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal and the silver enhancement reaction to magnify the detection signal. Moreover, gold nanoparticles (ANPs) play the roles of a label for antigen or antibody and a catalyst for silver precipitation. The change of the impedance to corroborate immuno-reaction is detected, amplified and analyzed. The results show that using the gap size of 20 μm electro-chip has the best sensitivity. The relationship between concentration and signal is established and the antigen detection limit is 100 ng/mL.

Original language	English
Pages	305-307
Number of pages	3
Publication status	Published - 2006 Jan 1
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 2006 Nov 5 → 2006 Nov 9

Other

Other	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	06-11-05 → 06-11-09

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

@conference{22e9210d5c764f61b6fe9cd779b12e9d,

title = "An electro-microchip for immunoassay using gold nanoparticle and silver enhancement",

abstract = "This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal and the silver enhancement reaction to magnify the detection signal. Moreover, gold nanoparticles (ANPs) play the roles of a label for antigen or antibody and a catalyst for silver precipitation. The change of the impedance to corroborate immuno-reaction is detected, amplified and analyzed. The results show that using the gap size of 20 μm electro-chip has the best sensitivity. The relationship between concentration and signal is established and the antigen detection limit is 100 ng/mL.",

author = "Huang, {Keng Shiang} and Huang, {Hao Hsuan} and Chang, {Tsung Chain} and Lin, {Hong Ping} and Chung, {Yung Chiang} and Lin, {Yu Cheng}",

year = "2006",

month = jan,

day = "1",

language = "English",

pages = "305--307",

note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

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TY - CONF

T1 - An electro-microchip for immunoassay using gold nanoparticle and silver enhancement

AU - Huang, Keng Shiang

AU - Huang, Hao Hsuan

AU - Chang, Tsung Chain

AU - Lin, Hong Ping

AU - Chung, Yung Chiang

AU - Lin, Yu Cheng

PY - 2006/1/1

Y1 - 2006/1/1

N2 - This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal and the silver enhancement reaction to magnify the detection signal. Moreover, gold nanoparticles (ANPs) play the roles of a label for antigen or antibody and a catalyst for silver precipitation. The change of the impedance to corroborate immuno-reaction is detected, amplified and analyzed. The results show that using the gap size of 20 μm electro-chip has the best sensitivity. The relationship between concentration and signal is established and the antigen detection limit is 100 ng/mL.

AB - This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal and the silver enhancement reaction to magnify the detection signal. Moreover, gold nanoparticles (ANPs) play the roles of a label for antigen or antibody and a catalyst for silver precipitation. The change of the impedance to corroborate immuno-reaction is detected, amplified and analyzed. The results show that using the gap size of 20 μm electro-chip has the best sensitivity. The relationship between concentration and signal is established and the antigen detection limit is 100 ng/mL.

UR - http://www.scopus.com/inward/record.url?scp=84902472031&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902472031&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902472031

SP - 305

EP - 307

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

An electro-microchip for immunoassay using gold nanoparticle and silver enhancement

Abstract

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All Science Journal Classification (ASJC) codes

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