An electro-microchip for immunoassay using gold nanoparticle and silver enhancement

Keng Shiang Huang, Hao Hsuan Huang, Tsung Chain Chang, Hong Ping Lin, Yung Chiang Chung, Yu Cheng Lin

Research output: Contribution to conferencePaperpeer-review

1 Citation (Scopus)

Abstract

This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal and the silver enhancement reaction to magnify the detection signal. Moreover, gold nanoparticles (ANPs) play the roles of a label for antigen or antibody and a catalyst for silver precipitation. The change of the impedance to corroborate immuno-reaction is detected, amplified and analyzed. The results show that using the gap size of 20 μm electro-chip has the best sensitivity. The relationship between concentration and signal is established and the antigen detection limit is 100 ng/mL.

Original languageEnglish
Pages305-307
Number of pages3
Publication statusPublished - 2006 Jan 1
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: 2006 Nov 52006 Nov 9

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period06-11-0506-11-09

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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