An electroporation microchip system for the transfection of zebrafish embryos using quantum dots and GFP genes for evaluation

Keng Shiang Huang, Yu-Cheng Lin, Kai Chun Su, Hung Yi Chen

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

This study focuses on the design and experimental verification of an electroporation (EP) microchip system for the transfection of zebrafish (Danio rerio). For generating suitable pulses, a circuit is used to provide voltages between 0 and 700 V, with nearly 0-3,500 V/cm electric field. In addition, a proposed EP microchip, designed in a modular fashion, is fabricated using micro electromechanical system (MEMS) technology to allow for rapid and convenient replacement of each component. A numerical simulation is carried out to analyze the uniformity and strength of the EP electric fields generated in the microchip. Trypan blue dye, water-soluble quantum dots (MUA-QDs) and genes coding for green fluorescence protein (pEGFP-N1 plasmids) were employed to verify the successful delivery and transfection of zebrafish embryos. The experimental results show that the optimum delivery rate of trypan blue dyes and MUA-QDs were respectively up to 62 and 36% by using the proposed EP system. The successfully transfected embryos with the pEGFP-N1 plasmid used exhibit green fluorescence in the zebrafish embryos. The approach in the transfection of zebrafish embryos will provide many potential usages for cellular imaging areas, gene therapy research and medical applications.

Original languageEnglish
Pages (from-to)761-768
Number of pages8
JournalBiomedical Microdevices
Volume9
Issue number5
DOIs
Publication statusPublished - 2007 Oct 1

Fingerprint

Quantum Dots
Electroporation
Zebrafish
Semiconductor quantum dots
Transfection
Embryonic Structures
Dyes
Genes
Fluorescence
Electric fields
Gene therapy
Medical applications
Trypan Blue
MEMS
Proteins
Imaging techniques
Plasmids
Coloring Agents
Networks (circuits)
Computer simulation

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Molecular Biology

Cite this

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An electroporation microchip system for the transfection of zebrafish embryos using quantum dots and GFP genes for evaluation. / Huang, Keng Shiang; Lin, Yu-Cheng; Su, Kai Chun; Chen, Hung Yi.

In: Biomedical Microdevices, Vol. 9, No. 5, 01.10.2007, p. 761-768.

Research output: Contribution to journalArticle

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