An electrospray ionization tandem mass spectrometry based system with an online dual-loop cleanup device for simultaneous quantitation of urinary benzene exposure biomarkers trans,trans-muconic acid and S-phenylmercapturic acid

Lung Cheng Lin, Jeng Fu Shih, Tung Sheng Shih, Yu Jang Li, Pao Chi Liao

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

An electrospray ionization tandem mass spectrometry (ESI-MS/MS) system with an online dual-loop cleanup device was developed for simultaneous quantitation of the urinary benzene exposure biomarkers trans,trans-muconic acid (ttMA) and S-phenylmercapturic acid (SPMA). The cleanup device was constructed from an autosampler, two electrically operated two-position switching valves, a reversed-phase C18 trap cartridge, a 200-μL loop, and two solvent-delivery pumps. The device was interfaced directly with a triple-quadrupole mass spectrometer and fully controlled by computer software and hardware. Because isotope dilution by introducing 13C-labeled ttMA and SPMA as internal standards was employed, the precision of the analytical system was high (for ttMA, intra- and inter-day CV values ranged from 3.82-4.53%; for SPMA, 2.13-7.06%). The calibration curves obtained using human urine spiked with ttMA were linear from 15.6-4000 μg/L (R = 0.9998) and SPMA at concentrations from 0.78-200 μg/L (R = 0.9993). The method detection limit (MDL) for SPMA was 0.23 μg/L. The MDL of ttMA could not be determined accurately because of unavailability of an appropriate blank urine matrix, but was estimated to be lower than 7.43 μg/L. Without tedious manual sample cleanup procedures the analytical system is fully automated and is therefore useful for high-throughput simultaneous determination of urinary ttMA and SPMA. The sample throughput is roughly 100 samples per day. With the selectivity and the sensitivity provided by MS/MS detection, the analytical system can be used for large-scale monitoring of environmental or occupational exposure of humans to benzene.

Original languageEnglish
Pages (from-to)2743-2752
Number of pages10
JournalRapid Communications in Mass Spectrometry
Volume18
Issue number22
DOIs
Publication statusPublished - 2004 Dec 6

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

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