@article{b25a0e7be362460eab68573da599eb7d,
title = "An iminocoumarin based covalent-assembly red-emitting fluorescent probe for detection of β-galactosidase activity in ovarian cancer cells",
abstract = "Upregulation of β-Galactosidase (β-Gal) has been generally found in primary ovarian cancer cells, making the intra-cellular β-Gal as a valuable biomarker for related tumors. In this study, we synthesized an iminocoumarin based covalent-assembly probe, Gal–IC–2, which has been designed to be activated by β-Gal and then rapidly afford to a fluorescent chromophore via a covalent-assembly mechanism with red emission for specific and sensitive detection of β-Gal activity. Gal–IC–2 showed significant fluorescence enhancement and a large Stokes shift in the presence of recombinant β-Gal, and the detection limit of this probe was 1.97 × 10−2 UmL−1. Notably, Gal–IC–2 also exhibits great stability in a broad pH range and is capable of detecting the reactivity of β-Gal in ovarian cancer cells. This is the first report, to the best of our knowledge, describing a covalent-assembly red-emitting probe for the detection and imaging of β-Gal both in vitro and in ovarian cancer cells.",
author = "Ku, {Ya Chu} and Lin, {Pin Han} and Huang, {Chung Yu} and Lee, {Chia Wei} and Yu, {Chien Hung} and Shih-Cheng Chen and Liu, {Wei Min}",
note = "Funding Information: This work was financially supported by the Ministry of Science and Technology, Taiwan [grant numbers: MOST 110-2113-M-030-006 to W.-M. Liu; MOST 111-2636-B-006-013 to C.-H. Yu). S.-C. Chen and C.-H. Yu are supported by the Young Scholar Fellowship Program by MOST in Taiwan. The authors gratefully acknowledge the Instrumentation Center of National Taiwan Normal University (High-Resolution Electrospray Mass Spectrometry/QSTAR{\textregistered} XL) and the Core Facility Center of National Cheng Kung University (MS004000 equipment) for their support and assistance in this work. Funding Information: This work was financially supported by the Ministry of Science and Technology, Taiwan [grant numbers: MOST 110-2113-M-030-006 to W.-M. Liu; MOST 111-2636-B-006-013 to C.-H. Yu). S.-C. Chen and C.-H. Yu are supported by the Young Scholar Fellowship Program by MOST in Taiwan. The authors gratefully acknowledge the Instrumentation Center of National Taiwan Normal University (High-Resolution Electrospray Mass Spectrometry/QSTAR{\textregistered} XL) and the Core Facility Center of National Cheng Kung University ( MS004000 equipment) for their support and assistance in this work. Publisher Copyright: {\textcopyright} 2022 Elsevier Ltd",
year = "2023",
month = feb,
doi = "10.1016/j.dyepig.2022.111004",
language = "English",
volume = "210",
journal = "Dyes and Pigments",
issn = "0143-7208",
publisher = "Elsevier BV",
}