An immunoassay using platinum nanoparticles, silver enhancement and a electrodes chips

Kuei Ling Su; I. Long Wang; Po Yu Tu; Yung Chiang Chung; Yu-Cheng Lin

An immunoassay using platinum nanoparticles, silver enhancement and a electrodes chips

Kuei Ling Su, I. Long Wang, Po Yu Tu, Yung Chiang Chung, Yu-Cheng Lin

Department of Engineering Science

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal and the silver enhancement reaction to magnify the detection signal. Moreover, platinum nanoparticles (PtNPs) play the roles of a label for antigen or antibody and a catalyst for silver precipitation. The change of the impedance to corroborate immuno-reaction is detected, amplified and analyzed. The developed method shows that a chip with a 50 μm length 150 μm electrodes gap size has the highest sensitivity. Therefore, using an electro-microchip, antibody-PtNPs conjugates and a silver enhancement reaction, a new immunoassay is successfully constructed.

Original language	English
Title of host publication	Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	1008-1011
Number of pages	4
ISBN (Print)	9780979806421
Publication status	Published - 2009 Jan 1
Event	13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of Duration: 2009 Nov 1 → 2009 Nov 5

Publication series

Name	Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other	13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Country	Korea, Republic of
City	Jeju
Period	09-11-01 → 09-11-05

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

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Su, K. L., Wang, I. L., Tu, P. Y., Chung, Y. C., & Lin, Y-C. (2009). An immunoassay using platinum nanoparticles, silver enhancement and a electrodes chips. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1008-1011). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

Su, Kuei Ling ; Wang, I. Long ; Tu, Po Yu ; Chung, Yung Chiang ; Lin, Yu-Cheng. / An immunoassay using platinum nanoparticles, silver enhancement and a electrodes chips. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. pp. 1008-1011 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

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title = "An immunoassay using platinum nanoparticles, silver enhancement and a electrodes chips",

abstract = "This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal and the silver enhancement reaction to magnify the detection signal. Moreover, platinum nanoparticles (PtNPs) play the roles of a label for antigen or antibody and a catalyst for silver precipitation. The change of the impedance to corroborate immuno-reaction is detected, amplified and analyzed. The developed method shows that a chip with a 50 μm length 150 μm electrodes gap size has the highest sensitivity. Therefore, using an electro-microchip, antibody-PtNPs conjugates and a silver enhancement reaction, a new immunoassay is successfully constructed.",

author = "Su, {Kuei Ling} and Wang, {I. Long} and Tu, {Po Yu} and Chung, {Yung Chiang} and Yu-Cheng Lin",

year = "2009",

month = jan,

day = "1",

language = "English",

isbn = "9780979806421",

series = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

pages = "1008--1011",

booktitle = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

note = "13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 ; Conference date: 01-11-2009 Through 05-11-2009",

}

Su, KL, Wang, IL, Tu, PY, Chung, YC & Lin, Y-C 2009, An immunoassay using platinum nanoparticles, silver enhancement and a electrodes chips. in Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 1008-1011, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, Jeju, Korea, Republic of, 09-11-01.

An immunoassay using platinum nanoparticles, silver enhancement and a electrodes chips. / Su, Kuei Ling; Wang, I. Long; Tu, Po Yu; Chung, Yung Chiang; Lin, Yu-Cheng.

Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 1008-1011 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - An immunoassay using platinum nanoparticles, silver enhancement and a electrodes chips

AU - Su, Kuei Ling

AU - Wang, I. Long

AU - Tu, Po Yu

AU - Chung, Yung Chiang

AU - Lin, Yu-Cheng

PY - 2009/1/1

Y1 - 2009/1/1

N2 - This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal and the silver enhancement reaction to magnify the detection signal. Moreover, platinum nanoparticles (PtNPs) play the roles of a label for antigen or antibody and a catalyst for silver precipitation. The change of the impedance to corroborate immuno-reaction is detected, amplified and analyzed. The developed method shows that a chip with a 50 μm length 150 μm electrodes gap size has the highest sensitivity. Therefore, using an electro-microchip, antibody-PtNPs conjugates and a silver enhancement reaction, a new immunoassay is successfully constructed.

AB - This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal and the silver enhancement reaction to magnify the detection signal. Moreover, platinum nanoparticles (PtNPs) play the roles of a label for antigen or antibody and a catalyst for silver precipitation. The change of the impedance to corroborate immuno-reaction is detected, amplified and analyzed. The developed method shows that a chip with a 50 μm length 150 μm electrodes gap size has the highest sensitivity. Therefore, using an electro-microchip, antibody-PtNPs conjugates and a silver enhancement reaction, a new immunoassay is successfully constructed.

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M3 - Conference contribution

AN - SCOPUS:84901811503

SN - 9780979806421

T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 1008

EP - 1011

BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009

Y2 - 1 November 2009 through 5 November 2009

ER -

Su KL, Wang IL, Tu PY, Chung YC, Lin Y-C. An immunoassay using platinum nanoparticles, silver enhancement and a electrodes chips. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2009. p. 1008-1011. (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).