TY - GEN
T1 - An immunoassay using platinum nanoparticles, silver enhancement and a electrodes chips
AU - Su, Kuei Ling
AU - Wang, I. Long
AU - Tu, Po Yu
AU - Chung, Yung Chiang
AU - Lin, Yu-Cheng
PY - 2009/1/1
Y1 - 2009/1/1
N2 - This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal and the silver enhancement reaction to magnify the detection signal. Moreover, platinum nanoparticles (PtNPs) play the roles of a label for antigen or antibody and a catalyst for silver precipitation. The change of the impedance to corroborate immuno-reaction is detected, amplified and analyzed. The developed method shows that a chip with a 50 μm length 150 μm electrodes gap size has the highest sensitivity. Therefore, using an electro-microchip, antibody-PtNPs conjugates and a silver enhancement reaction, a new immunoassay is successfully constructed.
AB - This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal and the silver enhancement reaction to magnify the detection signal. Moreover, platinum nanoparticles (PtNPs) play the roles of a label for antigen or antibody and a catalyst for silver precipitation. The change of the impedance to corroborate immuno-reaction is detected, amplified and analyzed. The developed method shows that a chip with a 50 μm length 150 μm electrodes gap size has the highest sensitivity. Therefore, using an electro-microchip, antibody-PtNPs conjugates and a silver enhancement reaction, a new immunoassay is successfully constructed.
UR - http://www.scopus.com/inward/record.url?scp=84901811503&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901811503&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901811503
SN - 9780979806421
T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1008
EP - 1011
BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Y2 - 1 November 2009 through 5 November 2009
ER -