An immunoassay using silver nanoparticle, silver enhancement and a flatbed scanner

Keng Shiang Huang, Wei Ting Chen, I. Long Wang, Hong Ping Lin, Tsung Chain Chang, Yu Cheng Lin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports a novel immunoassay using antibody-silver nanoparticles (Ab-AgNPs) conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, a flatbed scanner to scan and detect the immuno-reaction signal. This study is based on the sandwich immunoassay which contained a primary antibody, a secondary antibody and antigens. The results show that the silver precipitation was catalyzed by Ab-AgNPs conjugates. The changing color of reaction could be observed by naked eye or commercial scanner. And the detection limit was 1 ng/mL.

Original languageEnglish
Title of host publicationProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
EditorsJean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
PublisherChemical and Biological Microsystems Society
Pages62-64
Number of pages3
ISBN (Electronic)9780979806407
Publication statusPublished - 2007
Event11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France
Duration: 2007 Oct 72007 Oct 11

Publication series

NameProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

Conference

Conference11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
Country/TerritoryFrance
CityParis
Period07-10-0707-10-11

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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