An improved procedure for catheterization of the thoracic spinal subarachnoid space in the rat

Y. Y. Poon, Ya-Wen Zhang, S. F. Ko, Samuel H.H. Chan

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Catheterization of the subarachnoid space provides a convenient means to deliver drugs to, or collect cerebrospinal fluid from, the spinal cord in animal experiments, and has been instrumental to our understanding of spinal mechanisms that underlie anesthesia, analgesia, or cardiovascular regulation. Experience gained over the years has revealed several shortcomings of this technique. We report a procedure that encompasses the benefits of direct subarachnoid catheterization of the rat thoracic spinal cord but circumvents the known shortcomings. An intrathecal catheter was fabricated with a small silicon bead at one end of a PE-10 catheter, which was cannulated with a 4/0 suture that served as a guide. Using the L-shape hook of the suture guide as an anchorage, the catheter was advanced into the subarachnoid space until the silicon bead was lodged on a drilled hole (2 × 2 mm) over the lamina proper on the T13 vertebrae. With less surgical trauma, greater precision of placement and firmer anchorage of the catheter, less leakage of cerebrospinal fluid, and minimal mortality or morbidity, our modified procedure for catheterization of the thoracic spinal subarachnoid space in the rat compared favorably to previously reported methods.

Original languageEnglish
Pages (from-to)155-160
Number of pages6
JournalAnesthesia and analgesia
Volume101
Issue number1
DOIs
Publication statusPublished - 2005 Jan 1

Fingerprint

Subarachnoid Space
Catheterization
Thorax
Catheters
Silicon
Sutures
Spinal Cord
Anesthesia and Analgesia
Cerebrospinal Fluid
Spine
Morbidity
Mortality
Wounds and Injuries
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Anesthesiology and Pain Medicine

Cite this

Poon, Y. Y. ; Zhang, Ya-Wen ; Ko, S. F. ; Chan, Samuel H.H. / An improved procedure for catheterization of the thoracic spinal subarachnoid space in the rat. In: Anesthesia and analgesia. 2005 ; Vol. 101, No. 1. pp. 155-160.
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An improved procedure for catheterization of the thoracic spinal subarachnoid space in the rat. / Poon, Y. Y.; Zhang, Ya-Wen; Ko, S. F.; Chan, Samuel H.H.

In: Anesthesia and analgesia, Vol. 101, No. 1, 01.01.2005, p. 155-160.

Research output: Contribution to journalArticle

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