An in vitro monitoring system for simulated thermal process in cryosurgery

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

This paper describes a new in vitro experimental model that records temperature changes over a culture plate, which then can be used to assess the biological effects of cryosurgery. A cryoprobe and 16 thermocouples set up by a computer control system were used to monitor the temperature changes during freezing and thawing in a culture plate, and the data were used to create a temperature profile of the entire plate. Location of the thermocouples was confirmed by a digital camera viewing from under the plate, and temperature changes at any point in the interpolated areas were estimated using a curve fitting method. The estimated temperature was checked by sampling with four additional randomly placed thermocouples. Linear regression analysis showed that the estimated temperature and measured temperature were very close (correlation coefficients 0.98-0.99). MBT-2 tumor cells were cultured and then subjected to simulated cryosurgery. The surviving cells were stained with crystal violet and the cell death boundary was detected by image processing. Temperature history at the cell death boundary was retrieved and analyzed. With this system it is possible to recreate the temperature changes that result in a certain biological effect (such as cell death) during the process of simulated cryosurgery. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)159-170
Number of pages12
JournalCryobiology
Volume40
Issue number2
DOIs
Publication statusPublished - 2000 Mar

Fingerprint

Cryosurgery
cryosurgery
Hot Temperature
heat
Temperature
Monitoring
monitoring
thermocouples
temperature
Cell death
Thermocouples
cell death
Cell Death
gentian violet
Cultured Tumor Cells
Gentian Violet
In Vitro Techniques
Computer control systems
Thawing
Computer Systems

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

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title = "An in vitro monitoring system for simulated thermal process in cryosurgery",
abstract = "This paper describes a new in vitro experimental model that records temperature changes over a culture plate, which then can be used to assess the biological effects of cryosurgery. A cryoprobe and 16 thermocouples set up by a computer control system were used to monitor the temperature changes during freezing and thawing in a culture plate, and the data were used to create a temperature profile of the entire plate. Location of the thermocouples was confirmed by a digital camera viewing from under the plate, and temperature changes at any point in the interpolated areas were estimated using a curve fitting method. The estimated temperature was checked by sampling with four additional randomly placed thermocouples. Linear regression analysis showed that the estimated temperature and measured temperature were very close (correlation coefficients 0.98-0.99). MBT-2 tumor cells were cultured and then subjected to simulated cryosurgery. The surviving cells were stained with crystal violet and the cell death boundary was detected by image processing. Temperature history at the cell death boundary was retrieved and analyzed. With this system it is possible to recreate the temperature changes that result in a certain biological effect (such as cell death) during the process of simulated cryosurgery. (C) 2000 Academic Press.",
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An in vitro monitoring system for simulated thermal process in cryosurgery. / Yang, Wen Horng; Peng, Hsin Hsin; Chang, Hsien Chang; Shen, Shih Yu; Wu, Chao Liang; Chang, Chih Han.

In: Cryobiology, Vol. 40, No. 2, 03.2000, p. 159-170.

Research output: Contribution to journalArticle

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