The enhancement of our living quality has made people increase requirements of product functions. Bicycle-related clothing is known to play an important role for biking in comfort and safety. Most manufacturers mainly focus on the improvement of material properties of clothes instead of clothing design to meet customer requirements. This shortsighted view may not help increase the marketing competition. The objective of this research is to use an integrated procedure to determine customer requirements for bicycle leisure activity functional clothing design. In this research, a multiple regression analysis on identifying potential customers is used, and followed by the AIO lifestyle questionnaire, factor analysis and conjoint analysis to determine groups of customer preferences for bicycle leisure activity functional clothing design. The preferences of different ethnic group contents will be referenced for designers to define customer requirements and criteria in the process of bicycle leisure activity functional clothing development.