An integrated chip capable of performing sample pretreatment and nucleic acid amplification for HIV-1 detection

Jung Hao Wang, Lie Cheng, Chih Hung Wang, Wei Shuo Ling, Shainn Wei Wang, Gwo Bin Lee

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

This study reports on a microfluidic system equipped with a sample pretreatment device and a nucleic acid amplification device for the rapid diagnosis of the human immunodeficiency virus-1 (HIV-1). The system analyzed proviral deoxyribonucleic acid (DNA) from an HIV-infected Jurkat T cell line. In order to ensure accurate diagnosis among other prevalent B-type strains, simultaneous detections of four conserved HIV-1 B-type DNA fragments were performed in this integrated microfluidic system. The entire protocol including cell lysis, extraction of DNA, polymerase chain reaction (PCR), and optical detection were successfully integrated in order to perform a rapid, automated diagnosis. Experimental results showed that four primer sets with conserved HIV-1 B-type sequences specific for the 167-bp RU5 promoter region, the 424-bp int, the 117-bp tat, and the 162-bp vpr coding regions were successfully amplified from the respective regions of the proviral DNA, even from a single infected cell. This accurate real-time detection was achieved within 95. min using the integrated optical system.

Original languageEnglish
Pages (from-to)484-491
Number of pages8
JournalBiosensors and Bioelectronics
Volume41
Issue number1
DOIs
Publication statusPublished - 2013 Mar 15

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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