An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting

I. Fang Cheng, Hsien Chang Chang, Diana Hou, Hsueh Chia Chang

Research output: Contribution to journalArticlepeer-review

199 Citations (Scopus)

Abstract

Multi-target pathogen detection using heterogeneous medical samples require continuous filtering, sorting, and trapping of debris, bioparticles, and immunocolloids within a diagnostic chip. We present an integrated AC dielectrophoretic (DEP) microfluidic platform based on planar electrodes that form three-dimensional (3D) DEP gates. This platform can continuously perform these tasks with a throughput of 3 μmin. Mixtures of latex particles, Escherichia coli Nissle, Lactobacillus, and Candida albicans are sorted and concentrated by these 3D DEP gates. Surface enhanced Raman scattering is used as an on-chip detection method on the concentrated bacteria. A processing rate of 500 bacteria was estimated when 100 μl of a heterogeneous colony of 107 colony forming units/ml was processed in a single pass within 30 min.

Original languageEnglish
Article number021503
JournalBiomicrofluidics
Volume1
Issue number2
DOIs
Publication statusPublished - 2007

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Materials Science(all)
  • Genetics
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

Fingerprint Dive into the research topics of 'An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting'. Together they form a unique fingerprint.

Cite this