Abstract
The planar patch-clamp technique has revolutionized ion channel study by increasing throughput and minimizing sophisticated operation. Nevertheless, the system design is often focused toward a particular objective, which sometimes limits the range of information output. Here, we present an integrated electrophysiological and optical approach for ion channel study in a microfluidic system allowing multi-content detection. Multiple fluidic injections enable both intra- and extracellular solution exchange to facilitate the study of the ion channel in a microfluidic chip with high-yield seal formation. Whole-cell and single cell recordings were validated for various cell lines, i.e. endogenous channels of HIT-T15, CHO-K1, HEK-293T, and RIN-m5f cells. Long-term recording of temporal fluorescent changes on a trapped RIN-m5F cell shows the ability of extracellular solution exchange and simultaneous optical observation. The integrated microdevice system serves as a practical tool for high-quality and multi-content ion channel electrophysiological study.
| Original language | English |
|---|---|
| Pages (from-to) | 496-503 |
| Number of pages | 8 |
| Journal | Sensors and Actuators, B: Chemical |
| Volume | 185 |
| DOIs | |
| Publication status | Published - 2013 Jun 24 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Instrumentation
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry
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An integrated electrophysiological and optical approach for ion channel study in a microfluidic system enabling intra- and extra-cellular solution exchange. / Tu, Ting-Yuan; Chen, Chang Yu; Jong, De Shien; Wo, Andrew M.
In: Sensors and Actuators, B: Chemical, Vol. 185, 24.06.2013, p. 496-503.Research output: Contribution to journal › Article
TY - JOUR
T1 - An integrated electrophysiological and optical approach for ion channel study in a microfluidic system enabling intra- and extra-cellular solution exchange
AU - Tu, Ting-Yuan
AU - Chen, Chang Yu
AU - Jong, De Shien
AU - Wo, Andrew M.
PY - 2013/6/24
Y1 - 2013/6/24
N2 - The planar patch-clamp technique has revolutionized ion channel study by increasing throughput and minimizing sophisticated operation. Nevertheless, the system design is often focused toward a particular objective, which sometimes limits the range of information output. Here, we present an integrated electrophysiological and optical approach for ion channel study in a microfluidic system allowing multi-content detection. Multiple fluidic injections enable both intra- and extracellular solution exchange to facilitate the study of the ion channel in a microfluidic chip with high-yield seal formation. Whole-cell and single cell recordings were validated for various cell lines, i.e. endogenous channels of HIT-T15, CHO-K1, HEK-293T, and RIN-m5f cells. Long-term recording of temporal fluorescent changes on a trapped RIN-m5F cell shows the ability of extracellular solution exchange and simultaneous optical observation. The integrated microdevice system serves as a practical tool for high-quality and multi-content ion channel electrophysiological study.
AB - The planar patch-clamp technique has revolutionized ion channel study by increasing throughput and minimizing sophisticated operation. Nevertheless, the system design is often focused toward a particular objective, which sometimes limits the range of information output. Here, we present an integrated electrophysiological and optical approach for ion channel study in a microfluidic system allowing multi-content detection. Multiple fluidic injections enable both intra- and extracellular solution exchange to facilitate the study of the ion channel in a microfluidic chip with high-yield seal formation. Whole-cell and single cell recordings were validated for various cell lines, i.e. endogenous channels of HIT-T15, CHO-K1, HEK-293T, and RIN-m5f cells. Long-term recording of temporal fluorescent changes on a trapped RIN-m5F cell shows the ability of extracellular solution exchange and simultaneous optical observation. The integrated microdevice system serves as a practical tool for high-quality and multi-content ion channel electrophysiological study.
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U2 - 10.1016/j.snb.2013.05.036
DO - 10.1016/j.snb.2013.05.036
M3 - Article
VL - 185
SP - 496
EP - 503
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
ER -