An integrated electrophysiological and optical approach for ion channel study in a microfluidic system enabling intra- and extra-cellular solution exchange

Ting Yuan Tu, Chang Yu Chen, De Shien Jong, Andrew M. Wo

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The planar patch-clamp technique has revolutionized ion channel study by increasing throughput and minimizing sophisticated operation. Nevertheless, the system design is often focused toward a particular objective, which sometimes limits the range of information output. Here, we present an integrated electrophysiological and optical approach for ion channel study in a microfluidic system allowing multi-content detection. Multiple fluidic injections enable both intra- and extracellular solution exchange to facilitate the study of the ion channel in a microfluidic chip with high-yield seal formation. Whole-cell and single cell recordings were validated for various cell lines, i.e. endogenous channels of HIT-T15, CHO-K1, HEK-293T, and RIN-m5f cells. Long-term recording of temporal fluorescent changes on a trapped RIN-m5F cell shows the ability of extracellular solution exchange and simultaneous optical observation. The integrated microdevice system serves as a practical tool for high-quality and multi-content ion channel electrophysiological study.

Original languageEnglish
Pages (from-to)496-503
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume185
DOIs
Publication statusPublished - 2013 Jun 24

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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