An integrated electrophysiological and optical approach for ion channel study in a microfluidic system enabling intra- and extra-cellular solution exchange

Ting-Yuan Tu, Chang Yu Chen, De Shien Jong, Andrew M. Wo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The planar patch-clamp technique has revolutionized ion channel study by increasing throughput and minimizing sophisticated operation. Nevertheless, the system design is often focused toward a particular objective, which sometimes limits the range of information output. Here, we present an integrated electrophysiological and optical approach for ion channel study in a microfluidic system allowing multi-content detection. Multiple fluidic injections enable both intra- and extracellular solution exchange to facilitate the study of the ion channel in a microfluidic chip with high-yield seal formation. Whole-cell and single cell recordings were validated for various cell lines, i.e. endogenous channels of HIT-T15, CHO-K1, HEK-293T, and RIN-m5f cells. Long-term recording of temporal fluorescent changes on a trapped RIN-m5F cell shows the ability of extracellular solution exchange and simultaneous optical observation. The integrated microdevice system serves as a practical tool for high-quality and multi-content ion channel electrophysiological study.

Original languageEnglish
Pages (from-to)496-503
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume185
DOIs
Publication statusPublished - 2013 Jun 24

Fingerprint

Ion Channels
Microfluidics
Ion exchange
Ions
cells
recording
clamps
fluidics
Clamping devices
Fluidics
cultured cells
systems engineering
Seals
Systems analysis
chips
Cells
Throughput
injection
output

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

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An integrated electrophysiological and optical approach for ion channel study in a microfluidic system enabling intra- and extra-cellular solution exchange. / Tu, Ting-Yuan; Chen, Chang Yu; Jong, De Shien; Wo, Andrew M.

In: Sensors and Actuators, B: Chemical, Vol. 185, 24.06.2013, p. 496-503.

Research output: Contribution to journalArticle

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