TY - JOUR
T1 - An integrated microfluidic platform featuring real-time reverse transcription loop-mediated isothermal amplification for detection of COVID-19
AU - Jhou, You Ru
AU - Wang, Chih Hung
AU - Tsai, Huey Pin
AU - Shan, Yan Shen
AU - Lee, Gwo Bin
N1 - Funding Information:
The authors thank the Ministry of Science and Technology (MOST) of Taiwan funded this work (MOST 109B0247J3, 109-2224-E-007-002, 109-3114-Y-001-001, 109Q22803E1, & 110Q2804E1). Partial financial support from the National Health Research Institutes of Taiwan (NHRI-EX110-11020EI) is also greatly appreciated.
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/5/1
Y1 - 2022/5/1
N2 - An integrated microfluidic platform (IMP) utilizing real-time reverse-transcription loop-mediated isothermal amplification (RT-LAMP) was developed here for detection and quantification of three genes of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; i.e., coronavirus diseases 2019 (COVID-19)): RNA-dependent RNA polymerase, the envelope gene, and the nucleocapsid gene for molecular diagnosis. The IMP comprised a microfluidic chip, a temperature control module, a fluidic control module that collectively carried out viral lysis, RNA extraction, RT-LAMP, and the real-time detection within 90 min in an automatic format. A limit of detection of 5 × 103 copies/reaction for each gene was determined with three samples including synthesized RNAs, inactive viruses, and RNAs extracted from clinical samples; this compact platform could be a useful tool for COVID-19 diagnostics.
AB - An integrated microfluidic platform (IMP) utilizing real-time reverse-transcription loop-mediated isothermal amplification (RT-LAMP) was developed here for detection and quantification of three genes of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; i.e., coronavirus diseases 2019 (COVID-19)): RNA-dependent RNA polymerase, the envelope gene, and the nucleocapsid gene for molecular diagnosis. The IMP comprised a microfluidic chip, a temperature control module, a fluidic control module that collectively carried out viral lysis, RNA extraction, RT-LAMP, and the real-time detection within 90 min in an automatic format. A limit of detection of 5 × 103 copies/reaction for each gene was determined with three samples including synthesized RNAs, inactive viruses, and RNAs extracted from clinical samples; this compact platform could be a useful tool for COVID-19 diagnostics.
UR - http://www.scopus.com/inward/record.url?scp=85123625147&partnerID=8YFLogxK
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U2 - 10.1016/j.snb.2022.131447
DO - 10.1016/j.snb.2022.131447
M3 - Article
AN - SCOPUS:85123625147
VL - 358
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
SN - 0925-4005
M1 - 131447
ER -