An integrated microfluidic platform for cholangiocarcinoma diagnosis from clinical bile juice samples by utilizing multiple affinity reagents

Tsung Han Lu, Nai Jung Chiang, Chien Jui Huang, Priya Gopinathan, Hsiu Chi Tu, Yi Cheng Tsai, Yen Shen Shan, Shang Cheng Hung, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cholangiocarcinoma (CCA) is a lethal disease owing to its difficulty to be diagnosed at early stages and lack of effective treatment. To improve the accuracy of early diagnosis of CCA, one of the promising approaches is to use CCA-specific affinity reagents to detect CCA cells by liquid biopsy. In this study, bile juice, a body fluid close to lesions, was used as a clinical sample for CCA cell isolation and detection. Moreover, a novel microfluidic chip was designed and fabricated to detect CCA cells in the bile juice by using three types of affinity reagents. Experimental results showed that the three affinity reagents could capture CCA tumor cells in patients' bile juice (with a volume of only 3 mL) within 120 min. This microfluidic system may serve as a promising tool for detecting CCA in clinical settings.

Original languageEnglish
Title of host publication15th IEEE International Conference on Nano/Micro Engineered and Molecular System, NEMS 2020
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages261-264
Number of pages4
ISBN (Electronic)9781728172309
DOIs
Publication statusPublished - 2020 Sep 27
Event15th IEEE International Conference on Nano/Micro Engineered and Molecular System, NEMS 2020 - Virtual, San Diego, United States
Duration: 2020 Sep 272020 Sep 30

Publication series

Name15th IEEE International Conference on Nano/Micro Engineered and Molecular System, NEMS 2020

Conference

Conference15th IEEE International Conference on Nano/Micro Engineered and Molecular System, NEMS 2020
CountryUnited States
CityVirtual, San Diego
Period20-09-2720-09-30

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Electrochemistry
  • Biomedical Engineering
  • Electronic, Optical and Magnetic Materials
  • Instrumentation

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