An integrated microfluidic platform for rapid detection and subtyping of influenza viruses from clinical samples

Chien Hsuan Tai, Yi Che Tsai, Chih Hung Wang, Tzong Shiann Ho, Chih Peng Chang, Gwo Bin Lee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This study reports on the development of an integrated microfluidic system that performs sample pretreatment, nucleic acid amplification, and optical detection for molecular diagnosis of influenza viruses. The entire analysis protocol including virus lysis, extraction of ribonucleic acid, reverse transcription, polymerase chain reaction, and optical detection was successfully performed using the microfluidic system, which automatically performed the rapid diagnosis and subtyping of the influenza viruses. Signals obtained from an optical detection module could accurately differentiate influenza A/H1 (infA/H1), influenza A/H3 (infA/H3), influenza B (infB), and positive and negative control samples. More importantly, the entire process could be integrated and performed automatically with less human intervention. The experimental results demonstrated that this developed microfluidic system can successfully distinguish between infA/H1, infA/H3, and infB within 60 min. Furthermore, oral swabs from 92 patients were successfully tested using the developed microfluidic system. Thus, it may be a promising tool for rapid detection of novel and seasonal influenza strains in the near future.

Original languageEnglish
Pages (from-to)501-512
Number of pages12
JournalMicrofluidics and Nanofluidics
Volume16
Issue number3
DOIs
Publication statusPublished - 2014 Mar

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influenza
viruses
Viruses
Microfluidics
platforms
Polymerase chain reaction
Nucleic acids
Transcription
RNA
Nucleic Acids
Amplification
ribonucleic acids
polymerase chain reaction
nucleic acids
pretreatment
modules

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Materials Chemistry

Cite this

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abstract = "This study reports on the development of an integrated microfluidic system that performs sample pretreatment, nucleic acid amplification, and optical detection for molecular diagnosis of influenza viruses. The entire analysis protocol including virus lysis, extraction of ribonucleic acid, reverse transcription, polymerase chain reaction, and optical detection was successfully performed using the microfluidic system, which automatically performed the rapid diagnosis and subtyping of the influenza viruses. Signals obtained from an optical detection module could accurately differentiate influenza A/H1 (infA/H1), influenza A/H3 (infA/H3), influenza B (infB), and positive and negative control samples. More importantly, the entire process could be integrated and performed automatically with less human intervention. The experimental results demonstrated that this developed microfluidic system can successfully distinguish between infA/H1, infA/H3, and infB within 60 min. Furthermore, oral swabs from 92 patients were successfully tested using the developed microfluidic system. Thus, it may be a promising tool for rapid detection of novel and seasonal influenza strains in the near future.",
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An integrated microfluidic platform for rapid detection and subtyping of influenza viruses from clinical samples. / Tai, Chien Hsuan; Tsai, Yi Che; Wang, Chih Hung; Ho, Tzong Shiann; Chang, Chih Peng; Lee, Gwo Bin.

In: Microfluidics and Nanofluidics, Vol. 16, No. 3, 03.2014, p. 501-512.

Research output: Contribution to journalArticle

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