Abstract
Cholangiocarcinoma (CCA) is a very aggressive biliary tract malignancy and currently there is no reliable biomarker for diagnosis. In this work, an integrated microfluidic platform capable of performing cell-based systematic evolution of ligands by exponential enrichment (Cell SELEX) process with limited human intervention has been demonstrated for screening of aptamers specific to CCA. Our new magnetic-bead-based microfluidic platform is capable of reducing the time consumed to a 1/6th as compared to previous works. Promising aptamer candidates could be successfully selected in just 5 or 6 cycles as compared to the conventional 15+ cycles.
| Original language | English |
|---|---|
| Title of host publication | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 930-931 |
| Number of pages | 2 |
| ISBN (Electronic) | 9780979806490 |
| Publication status | Published - 2016 Jan 1 |
| Event | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 2016 Oct 9 → 2016 Oct 13 |
Publication series
| Name | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
|---|
Other
| Other | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
|---|---|
| Country | Ireland |
| City | Dublin |
| Period | 16-10-09 → 16-10-13 |
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All Science Journal Classification (ASJC) codes
- Control and Systems Engineering
Cite this
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An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process. / Gopinathan, Priya; Hung, Lien Yu; Wang, Chih Hung; Chiang, Nai Jung; Wang, Yu Chun; Shan, Yan Shen; Lee, Gwo Bin.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 930-931 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process
AU - Gopinathan, Priya
AU - Hung, Lien Yu
AU - Wang, Chih Hung
AU - Chiang, Nai Jung
AU - Wang, Yu Chun
AU - Shan, Yan Shen
AU - Lee, Gwo Bin
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Cholangiocarcinoma (CCA) is a very aggressive biliary tract malignancy and currently there is no reliable biomarker for diagnosis. In this work, an integrated microfluidic platform capable of performing cell-based systematic evolution of ligands by exponential enrichment (Cell SELEX) process with limited human intervention has been demonstrated for screening of aptamers specific to CCA. Our new magnetic-bead-based microfluidic platform is capable of reducing the time consumed to a 1/6th as compared to previous works. Promising aptamer candidates could be successfully selected in just 5 or 6 cycles as compared to the conventional 15+ cycles.
AB - Cholangiocarcinoma (CCA) is a very aggressive biliary tract malignancy and currently there is no reliable biomarker for diagnosis. In this work, an integrated microfluidic platform capable of performing cell-based systematic evolution of ligands by exponential enrichment (Cell SELEX) process with limited human intervention has been demonstrated for screening of aptamers specific to CCA. Our new magnetic-bead-based microfluidic platform is capable of reducing the time consumed to a 1/6th as compared to previous works. Promising aptamer candidates could be successfully selected in just 5 or 6 cycles as compared to the conventional 15+ cycles.
UR - http://www.scopus.com/inward/record.url?scp=85014147071&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85014147071&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85014147071
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 930
EP - 931
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
ER -