An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process

Priya Gopinathan, Lien Yu Hung, Chih Hung Wang, Nai Jung Chiang, Yu Chun Wang, Yan-Shen Shan, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cholangiocarcinoma (CCA) is a very aggressive biliary tract malignancy and currently there is no reliable biomarker for diagnosis. In this work, an integrated microfluidic platform capable of performing cell-based systematic evolution of ligands by exponential enrichment (Cell SELEX) process with limited human intervention has been demonstrated for screening of aptamers specific to CCA. Our new magnetic-bead-based microfluidic platform is capable of reducing the time consumed to a 1/6th as compared to previous works. Promising aptamer candidates could be successfully selected in just 5 or 6 cycles as compared to the conventional 15+ cycles.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages930-931
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016 Jan 1
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period16-10-0916-10-13

Fingerprint

Microfluidics
Screening
Biomarkers
Ligands

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Gopinathan, P., Hung, L. Y., Wang, C. H., Chiang, N. J., Wang, Y. C., Shan, Y-S., & Lee, G. B. (2016). An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 930-931). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.
Gopinathan, Priya ; Hung, Lien Yu ; Wang, Chih Hung ; Chiang, Nai Jung ; Wang, Yu Chun ; Shan, Yan-Shen ; Lee, Gwo Bin. / An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 930-931 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).
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title = "An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process",
abstract = "Cholangiocarcinoma (CCA) is a very aggressive biliary tract malignancy and currently there is no reliable biomarker for diagnosis. In this work, an integrated microfluidic platform capable of performing cell-based systematic evolution of ligands by exponential enrichment (Cell SELEX) process with limited human intervention has been demonstrated for screening of aptamers specific to CCA. Our new magnetic-bead-based microfluidic platform is capable of reducing the time consumed to a 1/6th as compared to previous works. Promising aptamer candidates could be successfully selected in just 5 or 6 cycles as compared to the conventional 15+ cycles.",
author = "Priya Gopinathan and Hung, {Lien Yu} and Wang, {Chih Hung} and Chiang, {Nai Jung} and Wang, {Yu Chun} and Yan-Shen Shan and Lee, {Gwo Bin}",
year = "2016",
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day = "1",
language = "English",
series = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",
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pages = "930--931",
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Gopinathan, P, Hung, LY, Wang, CH, Chiang, NJ, Wang, YC, Shan, Y-S & Lee, GB 2016, An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society, pp. 930-931, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 16-10-09.

An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process. / Gopinathan, Priya; Hung, Lien Yu; Wang, Chih Hung; Chiang, Nai Jung; Wang, Yu Chun; Shan, Yan-Shen; Lee, Gwo Bin.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 930-931 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process

AU - Gopinathan, Priya

AU - Hung, Lien Yu

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AU - Chiang, Nai Jung

AU - Wang, Yu Chun

AU - Shan, Yan-Shen

AU - Lee, Gwo Bin

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N2 - Cholangiocarcinoma (CCA) is a very aggressive biliary tract malignancy and currently there is no reliable biomarker for diagnosis. In this work, an integrated microfluidic platform capable of performing cell-based systematic evolution of ligands by exponential enrichment (Cell SELEX) process with limited human intervention has been demonstrated for screening of aptamers specific to CCA. Our new magnetic-bead-based microfluidic platform is capable of reducing the time consumed to a 1/6th as compared to previous works. Promising aptamer candidates could be successfully selected in just 5 or 6 cycles as compared to the conventional 15+ cycles.

AB - Cholangiocarcinoma (CCA) is a very aggressive biliary tract malignancy and currently there is no reliable biomarker for diagnosis. In this work, an integrated microfluidic platform capable of performing cell-based systematic evolution of ligands by exponential enrichment (Cell SELEX) process with limited human intervention has been demonstrated for screening of aptamers specific to CCA. Our new magnetic-bead-based microfluidic platform is capable of reducing the time consumed to a 1/6th as compared to previous works. Promising aptamer candidates could be successfully selected in just 5 or 6 cycles as compared to the conventional 15+ cycles.

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Gopinathan P, Hung LY, Wang CH, Chiang NJ, Wang YC, Shan Y-S et al. An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 930-931. (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).