An Integrated Microfluidic Platform to Detect FXYD2 RNA Expression in Ascites for Diagnosis of Ovarian Clear Cell Carcinoma

Yi Da Chung, Yuan Jhe Chuang, Chang Ni Lin, Keng Fu Hsu, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

An integrated microfluidic platform was developed to automate the entire procedures from cancer cell capturing in ascites, RNA extraction, and on-chip quantitative reverse-transcription polymerase chain reaction (qRT-PCR) for diagnosis of ovarian clear cell carcinoma (OCCC), which is a subtype of ovarian cancer (OvCa) with high mortality. FXYD2 gene expression was found highly associated with OCCC. A new protocol for detecting FXYD2 gene expression by referencing housekeeping gene was realized on the integrated microfluidic system. This detection method can be applied for diagnosis or prognosis monitoring of OCCC based on detection of the target gene expression level in liquid biopsy.

Original languageEnglish
Title of host publication34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages989-992
Number of pages4
ISBN (Electronic)9781665419123
DOIs
Publication statusPublished - 2021 Jan 25
Event34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021 - Virtual, Gainesville, United States
Duration: 2021 Jan 252021 Jan 29

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2021-January
ISSN (Print)1084-6999

Conference

Conference34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021
CountryUnited States
CityVirtual, Gainesville
Period21-01-2521-01-29

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'An Integrated Microfluidic Platform to Detect FXYD2 RNA Expression in Ascites for Diagnosis of Ovarian Clear Cell Carcinoma'. Together they form a unique fingerprint.

Cite this