An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers

Chen Hsun Weng; Lien Yu Hung; Hsin I. Lin; I. Shan Hsieh; Shu Chu Shiesh; Yu Ling Chen; Gwo Bin Lee

An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers

Chen Hsun Weng, Lien Yu Hung, Hsin I. Lin, I. Shan Hsieh, Shu Chu Shiesh, Yu Ling Chen, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A new microfluidic system involving a progressive selection of highly specific ligands by repeated rounds of partition and amplification from a large combinatorial nucleic acid library to screen tumor cell-specific aptamers was developed in this study. The systematic evolution of ligands by exponential enrichment (SELEX) process can be then automated. With this approach, an aptamer specific to lung cancer cells has been successfully screened. The entire process can be decreased from 2 weeks to only 3 days. The developed Cell-SELEX microsystem can be promising for fast screening of aptamers specific for tumor cells, which can be promising for early diagnosis of cancers and target therapy.

Original language	English
Title of host publication	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages	2086-2088
Number of pages	3
Volume	3
Publication status	Published - 2011
Event	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States Duration: 2011 Oct 2 → 2011 Oct 6

Other

Other	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/Territory	United States
City	Seattle, WA
Period	11-10-02 → 11-10-06

All Science Journal Classification (ASJC) codes

Control and Systems Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

@inproceedings{1942794123294bf6b9e29536ede74378,

title = "An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers",

abstract = "A new microfluidic system involving a progressive selection of highly specific ligands by repeated rounds of partition and amplification from a large combinatorial nucleic acid library to screen tumor cell-specific aptamers was developed in this study. The systematic evolution of ligands by exponential enrichment (SELEX) process can be then automated. With this approach, an aptamer specific to lung cancer cells has been successfully screened. The entire process can be decreased from 2 weeks to only 3 days. The developed Cell-SELEX microsystem can be promising for fast screening of aptamers specific for tumor cells, which can be promising for early diagnosis of cancers and target therapy.",

author = "Weng, {Chen Hsun} and Hung, {Lien Yu} and Lin, {Hsin I.} and Hsieh, {I. Shan} and Shiesh, {Shu Chu} and Chen, {Yu Ling} and Lee, {Gwo Bin}",

year = "2011",

language = "English",

isbn = "9781618395955",

volume = "3",

pages = "2086--2088",

booktitle = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",

note = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 ; Conference date: 02-10-2011 Through 06-10-2011",

}

Weng, CH, Hung, LY, Lin, HI, Hsieh, IS, Shiesh, SC, Chen, YL & Lee, GB 2011, An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. vol. 3, pp. 2086-2088, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 11-10-02.

An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers. / Weng, Chen Hsun; Hung, Lien Yu; Lin, Hsin I. et al.
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3 2011. p. 2086-2088.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers

AU - Weng, Chen Hsun

AU - Hung, Lien Yu

AU - Lin, Hsin I.

AU - Hsieh, I. Shan

AU - Shiesh, Shu Chu

AU - Chen, Yu Ling

AU - Lee, Gwo Bin

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AB - A new microfluidic system involving a progressive selection of highly specific ligands by repeated rounds of partition and amplification from a large combinatorial nucleic acid library to screen tumor cell-specific aptamers was developed in this study. The systematic evolution of ligands by exponential enrichment (SELEX) process can be then automated. With this approach, an aptamer specific to lung cancer cells has been successfully screened. The entire process can be decreased from 2 weeks to only 3 days. The developed Cell-SELEX microsystem can be promising for fast screening of aptamers specific for tumor cells, which can be promising for early diagnosis of cancers and target therapy.

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M3 - Conference contribution

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SN - 9781618395955

VL - 3

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EP - 2088

BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

Y2 - 2 October 2011 through 6 October 2011

ER -

An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers

Abstract

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