An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers

Chen Hsun Weng, Lien Yu Hung, Hsin I. Lin, I. Shan Hsieh, Shu Chu Shiesh, Yu Ling Chen, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

A new microfluidic system involving a progressive selection of highly specific ligands by repeated rounds of partition and amplification from a large combinatorial nucleic acid library to screen tumor cell-specific aptamers was developed in this study. The systematic evolution of ligands by exponential enrichment (SELEX) process can be then automated. With this approach, an aptamer specific to lung cancer cells has been successfully screened. The entire process can be decreased from 2 weeks to only 3 days. The developed Cell-SELEX microsystem can be promising for fast screening of aptamers specific for tumor cells, which can be promising for early diagnosis of cancers and target therapy.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages2086-2088
Number of pages3
Volume3
Publication statusPublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11-10-0211-10-06

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Microfluidics
Tumors
Ligands
Cells
Microsystems
Nucleic acids
Amplification
Screening

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Weng, C. H., Hung, L. Y., Lin, H. I., Hsieh, I. S., Shiesh, S. C., Chen, Y. L., & Lee, G. B. (2011). An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (Vol. 3, pp. 2086-2088)
Weng, Chen Hsun ; Hung, Lien Yu ; Lin, Hsin I. ; Hsieh, I. Shan ; Shiesh, Shu Chu ; Chen, Yu Ling ; Lee, Gwo Bin. / An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3 2011. pp. 2086-2088
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Weng, CH, Hung, LY, Lin, HI, Hsieh, IS, Shiesh, SC, Chen, YL & Lee, GB 2011, An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. vol. 3, pp. 2086-2088, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 11-10-02.

An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers. / Weng, Chen Hsun; Hung, Lien Yu; Lin, Hsin I.; Hsieh, I. Shan; Shiesh, Shu Chu; Chen, Yu Ling; Lee, Gwo Bin.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3 2011. p. 2086-2088.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Weng CH, Hung LY, Lin HI, Hsieh IS, Shiesh SC, Chen YL et al. An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3. 2011. p. 2086-2088