Abstract
A new microfluidic system involving a progressive selection of highly specific ligands by repeated rounds of partition and amplification from a large combinatorial nucleic acid library to screen tumor cell-specific aptamers was developed in this study. The systematic evolution of ligands by exponential enrichment (SELEX) process can be then automated. With this approach, an aptamer specific to lung cancer cells has been successfully screened. The entire process can be decreased from 2 weeks to only 3 days. The developed Cell-SELEX microsystem can be promising for fast screening of aptamers specific for tumor cells, which can be promising for early diagnosis of cancers and target therapy.
| Original language | English |
|---|---|
| Title of host publication | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
| Pages | 2086-2088 |
| Number of pages | 3 |
| Volume | 3 |
| Publication status | Published - 2011 |
| Event | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States Duration: 2011 Oct 2 → 2011 Oct 6 |
Other
| Other | 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 |
|---|---|
| Country | United States |
| City | Seattle, WA |
| Period | 11-10-02 → 11-10-06 |
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All Science Journal Classification (ASJC) codes
- Control and Systems Engineering
Cite this
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An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers. / Weng, Chen Hsun; Hung, Lien Yu; Lin, Hsin I.; Hsieh, I. Shan; Shiesh, Shu Chu; Chen, Yu Ling; Lee, Gwo Bin.
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 3 2011. p. 2086-2088.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers
AU - Weng, Chen Hsun
AU - Hung, Lien Yu
AU - Lin, Hsin I.
AU - Hsieh, I. Shan
AU - Shiesh, Shu Chu
AU - Chen, Yu Ling
AU - Lee, Gwo Bin
PY - 2011
Y1 - 2011
N2 - A new microfluidic system involving a progressive selection of highly specific ligands by repeated rounds of partition and amplification from a large combinatorial nucleic acid library to screen tumor cell-specific aptamers was developed in this study. The systematic evolution of ligands by exponential enrichment (SELEX) process can be then automated. With this approach, an aptamer specific to lung cancer cells has been successfully screened. The entire process can be decreased from 2 weeks to only 3 days. The developed Cell-SELEX microsystem can be promising for fast screening of aptamers specific for tumor cells, which can be promising for early diagnosis of cancers and target therapy.
AB - A new microfluidic system involving a progressive selection of highly specific ligands by repeated rounds of partition and amplification from a large combinatorial nucleic acid library to screen tumor cell-specific aptamers was developed in this study. The systematic evolution of ligands by exponential enrichment (SELEX) process can be then automated. With this approach, an aptamer specific to lung cancer cells has been successfully screened. The entire process can be decreased from 2 weeks to only 3 days. The developed Cell-SELEX microsystem can be promising for fast screening of aptamers specific for tumor cells, which can be promising for early diagnosis of cancers and target therapy.
UR - http://www.scopus.com/inward/record.url?scp=84874423281&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84874423281&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84874423281
SN - 9781618395955
VL - 3
SP - 2086
EP - 2088
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
ER -