An integrated microfluidic system for measurement of troponin I in whole blood by using a membrane-based assay on magnetic beads

Liang Ju Chien, Yu Ying Lin, Chi Han Chiou, Shiou Yi Kuo, Chih Jen Chen, Chih Chia Hsieh, Tzong Shiann Ho, Chen Hsun Weng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study will use a self-powered stream (STP) technique to develop a point-of-care cassette and based on the urgent need of the Acute myocardial infarction (AMI), we choose Troponin I as the detection target. The integration of the whole blood separation microfluidic cassette and magnetic control platform are used to complete the ultra-low concentration of biomarker. To achieve a high detection goal of the high sensitivity and accuracy of Troponin I, the study has developed three innovative modules: 1. To separate plasma from whole blood sample using the self-powered stream device 2. To locate the reagent at reagent well when plasma separation, a unique design that apply liquid-air surface tension to form the air-gate between reagents. 3. A Troponin I diagnostic cartridge with high sensitivity of 100 pg/mL, coefficient of variation (CV) ≤10%, and assay time ≤ 20 min.

Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages2303-2304
Number of pages2
ISBN (Electronic)9781510897571
Publication statusPublished - 2018 Jan 1
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan
Duration: 2018 Nov 112018 Nov 15

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume4

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Country/TerritoryTaiwan
CityKaohsiung
Period18-11-1118-11-15

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

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