An integrated microfluidic system for rapid, automatic and high-throughput staining of clinical tissue samples for diagnosis of ovarian cancer

Sheng Po Huang, Yuan Jhe Chuang, Wen Bin Lee, Yi Cheng Tsai, Chang Ni Lin, Keng Fu Hsu, Gwo Bin Lee

Research output: Contribution to journalArticle

Abstract

Accurate cancer diagnostic methods are of urgent need. Since traditional immunohistochemistry (IHC)-based approaches, while reliable, are labor-intensive and require well-trained technicians, we developed an integrated microfluidic platform capable of labeling ovarian cancer biomarkers (i.e. aptamer) within formalin-fixed, paraffin embedded tissues via molecular probes. Both aptamer-based 1) fluorescent staining and 2) IHC staining of clinical tissue samples could be automated in the microfluidic system in only 2-3 h (40-50% faster than conventional approaches) with <0.5 mL of reagents, signifying that this device could serve as a promising diagnostic tool for ovarian cancer.

Original languageEnglish
Pages (from-to)1103-1109
Number of pages7
JournalLab on a Chip
Volume20
Issue number6
DOIs
Publication statusPublished - 2020 Mar 21

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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