An integrated microfluidic system for rapid detection and multiple subtyping of influenza A viruses by using glycan-coated magnetic beads and RT-PCR

Kao Mai Shen, Narayana Murthy Sabbavarapu, Chien Yu Fu, Jia Tsrong Jan, Jen-Ren Wang, Shang Cheng Hung, Gwo Bin Lee

Research output: Contribution to journalArticle

Abstract

The influenza A (InfA) virus, which poses a significant global public health threat, is routinely classified into "subtypes" based on viral hemagglutinin (HA) and neuraminidase (NA) antigens. Because there are nearly 200 viral subtypes, current diagnostic approaches require multiplexing or array systems to cover various subtypes of HA and NA. A microfluidic chip featuring a HA × NA array was consequently developed herein for diagnosis and subtyping of InfA viruses via the use of glycan-coated magnetic beads followed by reverse transcription (RT) polymerase chain reaction (PCR). Up to 12 InfA subtypes were simultaneously detected in an automated fashion in less than 100 minutes on this microfluidic platform, representing a significant improvement in analysis speed compared to benchtop RT-PCR and chip-based microarray systems. The limits of detection of the RT-PCR assays ranged from 40 to 3000 copy numbers for the different subtypes of InfA viruses, around two orders of magnitude higher than in previous studies using microfluidic technologies. In summary, the array-type microfluidic chip system provides a rapid, sensitive, and fully automated approach for detection and multiple subtyping of InfA.

Original languageEnglish
Pages (from-to)1277-1286
Number of pages10
JournalLab on a Chip
Volume19
Issue number7
DOIs
Publication statusPublished - 2019 Jan 1

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Microfluidics
Polymerase chain reaction
Influenza A virus
Transcription
Viruses
Reverse Transcription
Polysaccharides
Neuraminidase
Polymerase Chain Reaction
Hemagglutinins
Human Influenza
Viral Hemagglutinins
Public health
Antigens
Microarrays
Multiplexing
Limit of Detection
Assays
Public Health
Technology

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Shen, Kao Mai ; Sabbavarapu, Narayana Murthy ; Fu, Chien Yu ; Jan, Jia Tsrong ; Wang, Jen-Ren ; Hung, Shang Cheng ; Lee, Gwo Bin. / An integrated microfluidic system for rapid detection and multiple subtyping of influenza A viruses by using glycan-coated magnetic beads and RT-PCR. In: Lab on a Chip. 2019 ; Vol. 19, No. 7. pp. 1277-1286.
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An integrated microfluidic system for rapid detection and multiple subtyping of influenza A viruses by using glycan-coated magnetic beads and RT-PCR. / Shen, Kao Mai; Sabbavarapu, Narayana Murthy; Fu, Chien Yu; Jan, Jia Tsrong; Wang, Jen-Ren; Hung, Shang Cheng; Lee, Gwo Bin.

In: Lab on a Chip, Vol. 19, No. 7, 01.01.2019, p. 1277-1286.

Research output: Contribution to journalArticle

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