An integrated microfluidic system for rapid, high-throughput staining of clinical tissue samples

Sheng Po Haung, Wen Bin Lee, Yi Cheng Tsai, Yuan Jhe Chuang, Keng Fu Hsu, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, we demonstrated an integrated microfluidic platform capable of performing the entire process for immunohistochemistry staining of formalin-fixed paraffin embedded (FFPE) tissue samples. The entire pretreatment and staining procedure could be automated within 2 hours, which is 50% shorter than the conventional approach. Moreover, ten tissue samples could be simultaneously stained by using a relatively small amount of reagents. A new aptamer specific to ovarian cancer was also used to replace conventional, expensive antibodies. With this approach, rapid and high-throughput staining of clinical tissue samples could be stained for cancer diagnosis.

Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages1163-1166
Number of pages4
ISBN (Electronic)9781510897571
Publication statusPublished - 2018 Jan 1
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan
Duration: 2018 Nov 112018 Nov 15

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume2

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
CountryTaiwan
CityKaohsiung
Period18-11-1118-11-15

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

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