An integrated microfluidic system for rapid screening of alpha-fetoprotein-specific aptamers

Chao Jyun Huang, Hsin I. Lin, Shu Chu Shiesh, Gwo Bin Lee

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The systematic evolution of ligands by exponential enrichment (SELEX) is a screening technique that involves the progressive selection of highly specific ligands via repeated rounds of partition and amplification from a large random pool of nucleic acid sequences. The products of this selection process are called aptamers and are either short single-stranded deoxyribonucleic acid (ssDNA) or ribonucleic acid (RNA) molecules with a high binding affinity to a large variety of target analytes. However, SELEX is a lengthy, labor-intensive, iterative process requiring multiple rounds of extraction and polymerase chain reaction (PCR) amplification. In order to address these problems, this study presents a new integrated microfluidic system consisting of a magnetic bead-based microfluidic SELEX chip and a competitive assay chip to automate the aptamer screening process. More importantly, the selected ssDNA sequences were confirmed to have a high affinity and specificity to the target molecules, using the developed competitive assay chip. With this approach, an aptamer specific to alpha-fetoprotein (AFP), which is a biomarker for liver cancers, has been successfully selected. The screened aptamer was used as a recognition molecule for AFP and has a linear detection range from 12.5 to 800. ng/mL, which was suitable for rapid clinical applications.

Original languageEnglish
Pages (from-to)50-55
Number of pages6
JournalBiosensors and Bioelectronics
Volume35
Issue number1
DOIs
Publication statusPublished - 2012 May 15

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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