An integrated microfluidic system for rapid screening of alpha-fetoprotein aptamers

Chao June Huang, Hsin I. Lin, Shu-Chu Shiesh, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Systematic evolution of ligands by exponential enrichment (SELEX) is a screening technique that involves a progressive selection of highly specific ligands by performing repeated rounds of partition and amplification from a large combinatorial nucleic acid library. The products of the selection are usually called aptamers, which are short single-stranded deoxyribonucleic acid (ssDNA) or ribonucleic acid (ssRNA) molecules binding with high affinity to a large variety of target analytes. However, SELEX is an iterative process requiring multiple rounds of extraction and polymerase chain reaction (PCR) amplification that demands significant sampling and process time. In order to address these problems, the study presents a new integrated microfluidic system consisting of an automatic SELEX chip and a competitive assay chip to enhance the aptamer screening efficiency. With this approach, an aptamer specific to alpha-fetoprotein (AFP), which is a biomarker for liver cancer, has been successfully screened.

Original languageEnglish
Title of host publication2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011
Pages185-188
Number of pages4
DOIs
Publication statusPublished - 2011 Apr 13
Event24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011 - Cancun, Mexico
Duration: 2011 Jan 232011 Jan 27

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011
CountryMexico
CityCancun
Period11-01-2311-01-27

Fingerprint

alpha-Fetoproteins
Microfluidics
Screening
screening
Ligands
ligands
Amplification
chips
ribonucleic acids
polymerase chain reaction
biomarkers
Polymerase chain reaction
Nucleic acids
nucleic acids
Biomarkers
RNA
liver
Liver
Nucleic Acids
affinity

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Huang, C. J., Lin, H. I., Shiesh, S-C., & Lee, G. B. (2011). An integrated microfluidic system for rapid screening of alpha-fetoprotein aptamers. In 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011 (pp. 185-188). [5734392] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2011.5734392
Huang, Chao June ; Lin, Hsin I. ; Shiesh, Shu-Chu ; Lee, Gwo Bin. / An integrated microfluidic system for rapid screening of alpha-fetoprotein aptamers. 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011. 2011. pp. 185-188 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).
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Huang, CJ, Lin, HI, Shiesh, S-C & Lee, GB 2011, An integrated microfluidic system for rapid screening of alpha-fetoprotein aptamers. in 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011., 5734392, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 185-188, 24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011, Cancun, Mexico, 11-01-23. https://doi.org/10.1109/MEMSYS.2011.5734392

An integrated microfluidic system for rapid screening of alpha-fetoprotein aptamers. / Huang, Chao June; Lin, Hsin I.; Shiesh, Shu-Chu; Lee, Gwo Bin.

2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011. 2011. p. 185-188 5734392 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Huang CJ, Lin HI, Shiesh S-C, Lee GB. An integrated microfluidic system for rapid screening of alpha-fetoprotein aptamers. In 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011. 2011. p. 185-188. 5734392. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2011.5734392