TY - GEN
T1 - An integrated microfluidic system for rapid screening of alpha-fetoprotein aptamers
AU - Huang, Chao June
AU - Lin, Hsin I.
AU - Shiesh, Shu-Chu
AU - Lee, Gwo Bin
PY - 2011/4/13
Y1 - 2011/4/13
N2 - Systematic evolution of ligands by exponential enrichment (SELEX) is a screening technique that involves a progressive selection of highly specific ligands by performing repeated rounds of partition and amplification from a large combinatorial nucleic acid library. The products of the selection are usually called aptamers, which are short single-stranded deoxyribonucleic acid (ssDNA) or ribonucleic acid (ssRNA) molecules binding with high affinity to a large variety of target analytes. However, SELEX is an iterative process requiring multiple rounds of extraction and polymerase chain reaction (PCR) amplification that demands significant sampling and process time. In order to address these problems, the study presents a new integrated microfluidic system consisting of an automatic SELEX chip and a competitive assay chip to enhance the aptamer screening efficiency. With this approach, an aptamer specific to alpha-fetoprotein (AFP), which is a biomarker for liver cancer, has been successfully screened.
AB - Systematic evolution of ligands by exponential enrichment (SELEX) is a screening technique that involves a progressive selection of highly specific ligands by performing repeated rounds of partition and amplification from a large combinatorial nucleic acid library. The products of the selection are usually called aptamers, which are short single-stranded deoxyribonucleic acid (ssDNA) or ribonucleic acid (ssRNA) molecules binding with high affinity to a large variety of target analytes. However, SELEX is an iterative process requiring multiple rounds of extraction and polymerase chain reaction (PCR) amplification that demands significant sampling and process time. In order to address these problems, the study presents a new integrated microfluidic system consisting of an automatic SELEX chip and a competitive assay chip to enhance the aptamer screening efficiency. With this approach, an aptamer specific to alpha-fetoprotein (AFP), which is a biomarker for liver cancer, has been successfully screened.
UR - http://www.scopus.com/inward/record.url?scp=79953781053&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79953781053&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2011.5734392
DO - 10.1109/MEMSYS.2011.5734392
M3 - Conference contribution
AN - SCOPUS:79953781053
SN - 9781424496327
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 185
EP - 188
BT - 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011
T2 - 24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011
Y2 - 23 January 2011 through 27 January 2011
ER -
