An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology

Ching Wen Yu, Lien Yu Hung, Chien Yu Fu, Chih Hung Wang, Nai Jung Chiang, Yu Chun Wang, Yan Shen Shan, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Cholangiocarcinoma (CCA) is a cancer of bile duct, which possesses a high mortality rate and poor prognosis owing to its difficulties in early diagnosis and a lack of effective treatment. To improve the clinical detection, one of promising approaches is to find specific biomarkers to detect CCA cells. However, the existing screening processes are usually time-consuming and lab-intensive. In this study, an integrated microfluidic system was proposed to perform on-chip phage display for selection of specific peptides that could identify CCA cell lines. The results showed that we could perform one panning round within 6 hours and the whole processes could be automated. This microfluidic system may be a powerful tool for screening of affinity agents in the clinical diagnosis and target therapeutics for CCA.

Original languageEnglish
Title of host publication2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages561-564
Number of pages4
ISBN (Electronic)9781509019472
DOIs
Publication statusPublished - 2016 Nov 28
Event11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 - Sendai, Japan
Duration: 2016 Apr 172016 Apr 20

Publication series

Name2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

Other

Other11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
CountryJapan
CitySendai
Period16-04-1716-04-20

All Science Journal Classification (ASJC) codes

  • Industrial and Manufacturing Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Electronic, Optical and Magnetic Materials

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