An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology

Ching Wen Yu; Lien Yu Hung; Chien Yu Fu; Chih Hung Wang; Nai Jung Chiang; Yu Chun Wang; Yan Shen Shan; Gwo Bin Lee

doi:10.1109/NEMS.2016.7758314

An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology

Ching Wen Yu
, Lien Yu Hung
, Chien Yu Fu
, Chih Hung Wang
, Nai Jung Chiang
, Yu Chun Wang
, Yan Shen Shan
, Gwo Bin Lee

Institute of Clinical Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

Cholangiocarcinoma (CCA) is a cancer of bile duct, which possesses a high mortality rate and poor prognosis owing to its difficulties in early diagnosis and a lack of effective treatment. To improve the clinical detection, one of promising approaches is to find specific biomarkers to detect CCA cells. However, the existing screening processes are usually time-consuming and lab-intensive. In this study, an integrated microfluidic system was proposed to perform on-chip phage display for selection of specific peptides that could identify CCA cell lines. The results showed that we could perform one panning round within 6 hours and the whole processes could be automated. This microfluidic system may be a powerful tool for screening of affinity agents in the clinical diagnosis and target therapeutics for CCA.

Original language	English
Title of host publication	2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	561-564
Number of pages	4
ISBN (Electronic)	9781509019472
DOIs	https://doi.org/10.1109/NEMS.2016.7758314
Publication status	Published - 2016 Nov 28
Event	11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 - Sendai, Japan Duration: 2016 Apr 17 → 2016 Apr 20

Publication series

Name	2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

Other

Other	11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
Country/Territory	Japan
City	Sendai
Period	16-04-17 → 16-04-20

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Industrial and Manufacturing Engineering
Mechanical Engineering
Mechanics of Materials
Electronic, Optical and Magnetic Materials

Access to Document

10.1109/NEMS.2016.7758314

Cite this

Yu, C. W., Hung, L. Y., Fu, C. Y., Wang, C. H., Chiang, N. J., Wang, Y. C., Shan, Y. S., & Lee, G. B. (2016). An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology. In 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 (pp. 561-564). Article 7758314 (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2016.7758314

Yu, Ching Wen ; Hung, Lien Yu ; Fu, Chien Yu et al. / An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology. 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc., 2016. pp. 561-564 (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016).

@inproceedings{e85e087bc62a449e979368be3341a85f,

title = "An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology",

abstract = "Cholangiocarcinoma (CCA) is a cancer of bile duct, which possesses a high mortality rate and poor prognosis owing to its difficulties in early diagnosis and a lack of effective treatment. To improve the clinical detection, one of promising approaches is to find specific biomarkers to detect CCA cells. However, the existing screening processes are usually time-consuming and lab-intensive. In this study, an integrated microfluidic system was proposed to perform on-chip phage display for selection of specific peptides that could identify CCA cell lines. The results showed that we could perform one panning round within 6 hours and the whole processes could be automated. This microfluidic system may be a powerful tool for screening of affinity agents in the clinical diagnosis and target therapeutics for CCA.",

author = "Yu, \{Ching Wen\} and Hung, \{Lien Yu\} and Fu, \{Chien Yu\} and Wang, \{Chih Hung\} and Chiang, \{Nai Jung\} and Wang, \{Yu Chun\} and Shan, \{Yan Shen\} and Lee, \{Gwo Bin\}",

note = "Funding Information: The authors would like to thank financial supports from Ministry of Science and Technology, Taiwan (104- 2119-M-007-009, 104-2811-E-007-031) and {"}Towards a World-Class University{"} Project (104N2751E1). Partial financial support from NHRI, Taiwan (NHRI-EX104- 10428EI) is greatly appreciated. Publisher Copyright: {\textcopyright} 2016 IEEE.; 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 ; Conference date: 17-04-2016 Through 20-04-2016",

year = "2016",

month = nov,

day = "28",

doi = "10.1109/NEMS.2016.7758314",

language = "English",

series = "2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "561--564",

booktitle = "2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016",

address = "United States",

}

Yu, CW, Hung, LY, Fu, CY, Wang, CH, Chiang, NJ, Wang, YC, Shan, YS & Lee, GB 2016, An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology. in 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016., 7758314, 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016, Institute of Electrical and Electronics Engineers Inc., pp. 561-564, 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016, Sendai, Japan, 16-04-17. https://doi.org/10.1109/NEMS.2016.7758314

An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology. / Yu, Ching Wen; Hung, Lien Yu; Fu, Chien Yu et al.
2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc., 2016. p. 561-564 7758314 (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology

AU - Yu, Ching Wen

AU - Hung, Lien Yu

AU - Fu, Chien Yu

AU - Wang, Chih Hung

AU - Chiang, Nai Jung

AU - Wang, Yu Chun

AU - Shan, Yan Shen

AU - Lee, Gwo Bin

N1 - Funding Information: The authors would like to thank financial supports from Ministry of Science and Technology, Taiwan (104- 2119-M-007-009, 104-2811-E-007-031) and "Towards a World-Class University" Project (104N2751E1). Partial financial support from NHRI, Taiwan (NHRI-EX104- 10428EI) is greatly appreciated. Publisher Copyright: © 2016 IEEE.

PY - 2016/11/28

Y1 - 2016/11/28

N2 - Cholangiocarcinoma (CCA) is a cancer of bile duct, which possesses a high mortality rate and poor prognosis owing to its difficulties in early diagnosis and a lack of effective treatment. To improve the clinical detection, one of promising approaches is to find specific biomarkers to detect CCA cells. However, the existing screening processes are usually time-consuming and lab-intensive. In this study, an integrated microfluidic system was proposed to perform on-chip phage display for selection of specific peptides that could identify CCA cell lines. The results showed that we could perform one panning round within 6 hours and the whole processes could be automated. This microfluidic system may be a powerful tool for screening of affinity agents in the clinical diagnosis and target therapeutics for CCA.

AB - Cholangiocarcinoma (CCA) is a cancer of bile duct, which possesses a high mortality rate and poor prognosis owing to its difficulties in early diagnosis and a lack of effective treatment. To improve the clinical detection, one of promising approaches is to find specific biomarkers to detect CCA cells. However, the existing screening processes are usually time-consuming and lab-intensive. In this study, an integrated microfluidic system was proposed to perform on-chip phage display for selection of specific peptides that could identify CCA cell lines. The results showed that we could perform one panning round within 6 hours and the whole processes could be automated. This microfluidic system may be a powerful tool for screening of affinity agents in the clinical diagnosis and target therapeutics for CCA.

UR - https://www.scopus.com/pages/publications/85007256358

UR - https://www.scopus.com/pages/publications/85007256358#tab=citedBy

U2 - 10.1109/NEMS.2016.7758314

DO - 10.1109/NEMS.2016.7758314

M3 - Conference contribution

AN - SCOPUS:85007256358

T3 - 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

SP - 561

EP - 564

BT - 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

Y2 - 17 April 2016 through 20 April 2016

ER -

Yu CW, Hung LY, Fu CY, Wang CH, Chiang NJ, Wang YC et al. An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology. In 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc. 2016. p. 561-564. 7758314. (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016). doi: 10.1109/NEMS.2016.7758314

An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology

Abstract

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UN SDGs

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