An integrated microfluidic system using buried optical fibers for detection of phalaenopsis orchid pathogens

Chih Lin Lin, Wen Hsin Chang, Chih Hung Wang, Ping Chen Li, Fuh Jyh Jan, Tzong-Yueh Chen, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

There is a critical need for early detection of pathogens in Phalaenopsis orchid due to its high economic value in agriculture. In our previous work [1], an integrated microfluidic system capable of detecting these pathogens through pathogen-specific ribonucleic acid (RNA) purification and RNA amplification by using reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) was presented. In this study, a buried optical fiber-based detection module was integrated with the microfluidic chip to monitor the RT-LAMP reaction directly on-chip such that in-line optical detection can be realized. Successful RT-LAMP reaction, which amplifies the nucleic acids that yield the precipitate, magnesium pyrophosphate, and therefore increased the turbidity in the LAMP products could be detected optically. Furthermore, a fluorescent dye, calcein, could be also used in our system to detect the fluorescent signals, if necessary. Thus sensitive and fast diagnosis of the pathogens in Phalaenopsis orchids could be successfully achieved by using the integrated microfluidic system.

Original languageEnglish
Title of host publication2013 Transducers and Eurosensors XXVII
Subtitle of host publicationThe 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Pages1231-1234
Number of pages4
DOIs
Publication statusPublished - 2013 Dec 1
Event2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 - Barcelona, Spain
Duration: 2013 Jun 162013 Jun 20

Publication series

Name2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

Other

Other2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
CountrySpain
CityBarcelona
Period13-06-1613-06-20

All Science Journal Classification (ASJC) codes

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'An integrated microfluidic system using buried optical fibers for detection of phalaenopsis orchid pathogens'. Together they form a unique fingerprint.

Cite this