An integrated microfluidic system with field-effect-transistor sensor arrays for detecting multiple cardiovascular biomarkers from clinical samples

Anirban Sinha, Tse Yu Tai, Kuang Hsien Li, Priya Gopinathan, Yi Da Chung, Indu Sarangadharan, Hsi Pin Ma, Po Chiun Huang, Shu-Chu Shiesh, Yu Lin Wang, Gwo Bin Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Certain blood-borne biomarkers offer a potent methodology for understanding the risk of cardiovascular diseases (CVDs) with clinicians generally advocating the use of multiple biomarkers for proper risk assessment of CVDs. Herein four such CVDs biomarkers- C-reactive protein (CRP), N-terminal pro b-type natriuretic peptide (NT-proBNP), cardiac troponin I (cTnI), and fibrinogen- were rapidly (5 min) analyzed from clinical samples (~ 4 µL) on an integrated microfluidic platform equipped with 1) immobilized highly specific aptamer probes and 2) field-effect transistor (FET)-based sensor arrays. The calibration curve from the FET sensor arrays showed good agreement in the physiological concentration ranges for CRP (0.1–50 mg/L), NT-proBNP (50–10,000 pg/mL), cTnI (1–10,000 pg/mL), and fibrinogen (0.1–5 mg/mL). The developed prototype of this fully automated portable device requires minimal reagent and sample inputs and consequently shows great promise for next-generation point-of-care devices assaying multiple CVDs biomarkers in clinical samples.

Original languageEnglish
Pages (from-to)155-163
Number of pages9
JournalBiosensors and Bioelectronics
Volume129
DOIs
Publication statusPublished - 2019 Mar 15

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Microfluidics
Sensor arrays
Biomarkers
Field effect transistors
Cardiovascular Diseases
Natriuretic Peptides
Troponin I
C-Reactive Protein
Fibrinogen
Point-of-Care Systems
Proteins
Equipment and Supplies
Risk assessment
Calibration
Blood

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Sinha, Anirban ; Tai, Tse Yu ; Li, Kuang Hsien ; Gopinathan, Priya ; Chung, Yi Da ; Sarangadharan, Indu ; Ma, Hsi Pin ; Huang, Po Chiun ; Shiesh, Shu-Chu ; Wang, Yu Lin ; Lee, Gwo Bin. / An integrated microfluidic system with field-effect-transistor sensor arrays for detecting multiple cardiovascular biomarkers from clinical samples. In: Biosensors and Bioelectronics. 2019 ; Vol. 129. pp. 155-163.
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An integrated microfluidic system with field-effect-transistor sensor arrays for detecting multiple cardiovascular biomarkers from clinical samples. / Sinha, Anirban; Tai, Tse Yu; Li, Kuang Hsien; Gopinathan, Priya; Chung, Yi Da; Sarangadharan, Indu; Ma, Hsi Pin; Huang, Po Chiun; Shiesh, Shu-Chu; Wang, Yu Lin; Lee, Gwo Bin.

In: Biosensors and Bioelectronics, Vol. 129, 15.03.2019, p. 155-163.

Research output: Contribution to journalArticle

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AU - Sarangadharan, Indu

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AU - Wang, Yu Lin

AU - Lee, Gwo Bin

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