An Integrated Platform for Serological Detection and Vaccination of COVID-19

Sung Chan Wei, Wei Ting Hsu, Chun Hsiang Chiu, Feng Yee Chang, Huei Ru Lo, Chuan Yu Liao, Hwai I. Yang, Yu Chi Chou, Chih Hsuan Tsai, Yu Chan Chao

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Coronavirus Disease 2019 (COVID-19), caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is an ongoing pandemic. Detection and vaccination are essential for disease control, but they are distinct and complex operations that require significant improvements. Here, we developed an integrated detection and vaccination system to greatly simplify these efforts. We constructed recombinant baculoviruses to separately display the nucleocapsid (N) and spike (S) proteins of SARS-CoV-2. Insect cells infected by the recombinant baculoviruses were used to generate a cell-based system to accurately detect patient serum. Notably, although well-recognized by our newly developed detection system in which S-displaying insect cells acted as antigen, anti-S antibodies from many patients were barely detectable by Western blot, evidencing that COVID-19 patients primarily produce conformation-dependent anti-S antibodies. Furthermore, the same baculovirus constructs can display N (N-Bac) or S (S-Bac) on the baculovirus envelope and serve as vector vaccines. Animal experiments show that S-Bac or N-Bac immunization in mice elicited a strong and specific antibody response, and S-Bac in particular stimulated effective neutralizing antibodies without the need for adjuvant. Our integrated system maintains antigen conformation and membrane structure to facilitate serum detection and antibody stimulation. Thus, compared with currently available technologies, our system represents a simplified and efficient platform for better SARS-CoV-2 detection and vaccination.

Original languageEnglish
Article number771011
JournalFrontiers in Immunology
Volume12
DOIs
Publication statusPublished - 2021 Dec 23

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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