An integrated system of RBCs sorting and Brownian motion bio-sensing technique

Yu Jui Fan, Ching Jiun Lee, Jen Hao Hsu, Yi Hsing Liu, Szu-I Yeh, Horn Jiunn Sheen, Shiming Lin, Jing Fa Tsai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A clinical sorting chip integrated with new protein sensing technique has been developed in this work. Antibody-coated nanobeads reacted with antigen was separated from blood plasma based on dielectrophoresis (DEP) forces. MEMS fabrication process was used to produce the bio-chip and only two photo masks were required in the experiment. By using oxygen-plasma direct bonding method, the assembly of an electrode chip and the microchannels will form a set of RBCs and nanobeads separating chip. The original concentration of antigen can be rapidly estimated by nanobeads' Brownian motion measurement in varies of the viscous solutions. During the antibody-antigen reacting, the mean value of the beads diameter increased so that the Brownian velocity decreased with increasing time. The Brownian motion of nanobeads was observed and measured by micro-particle tracking velocimetry (μ-PTV).

Original languageEnglish
Title of host publication2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
Pages487-491
Number of pages5
DOIs
Publication statusPublished - 2010 Nov 29
Event5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010 - Xiamen, China
Duration: 2010 Jan 202010 Jan 23

Publication series

Name2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010

Other

Other5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
CountryChina
CityXiamen
Period10-01-2010-01-23

Fingerprint

Brownian movement
Antigens
Sorting
Antibodies
Plasmas
Microchannels
Electrophoresis
Velocity measurement
MEMS
Masks
Blood
Proteins
Fabrication
Electrodes
Oxygen
Experiments

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

Cite this

Fan, Y. J., Lee, C. J., Hsu, J. H., Liu, Y. H., Yeh, S-I., Sheen, H. J., ... Tsai, J. F. (2010). An integrated system of RBCs sorting and Brownian motion bio-sensing technique. In 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010 (pp. 487-491). [5592441] (2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010). https://doi.org/10.1109/NEMS.2010.5592441
Fan, Yu Jui ; Lee, Ching Jiun ; Hsu, Jen Hao ; Liu, Yi Hsing ; Yeh, Szu-I ; Sheen, Horn Jiunn ; Lin, Shiming ; Tsai, Jing Fa. / An integrated system of RBCs sorting and Brownian motion bio-sensing technique. 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010. 2010. pp. 487-491 (2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010).
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abstract = "A clinical sorting chip integrated with new protein sensing technique has been developed in this work. Antibody-coated nanobeads reacted with antigen was separated from blood plasma based on dielectrophoresis (DEP) forces. MEMS fabrication process was used to produce the bio-chip and only two photo masks were required in the experiment. By using oxygen-plasma direct bonding method, the assembly of an electrode chip and the microchannels will form a set of RBCs and nanobeads separating chip. The original concentration of antigen can be rapidly estimated by nanobeads' Brownian motion measurement in varies of the viscous solutions. During the antibody-antigen reacting, the mean value of the beads diameter increased so that the Brownian velocity decreased with increasing time. The Brownian motion of nanobeads was observed and measured by micro-particle tracking velocimetry (μ-PTV).",
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Fan, YJ, Lee, CJ, Hsu, JH, Liu, YH, Yeh, S-I, Sheen, HJ, Lin, S & Tsai, JF 2010, An integrated system of RBCs sorting and Brownian motion bio-sensing technique. in 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010., 5592441, 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010, pp. 487-491, 5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010, Xiamen, China, 10-01-20. https://doi.org/10.1109/NEMS.2010.5592441

An integrated system of RBCs sorting and Brownian motion bio-sensing technique. / Fan, Yu Jui; Lee, Ching Jiun; Hsu, Jen Hao; Liu, Yi Hsing; Yeh, Szu-I; Sheen, Horn Jiunn; Lin, Shiming; Tsai, Jing Fa.

2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010. 2010. p. 487-491 5592441 (2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Fan YJ, Lee CJ, Hsu JH, Liu YH, Yeh S-I, Sheen HJ et al. An integrated system of RBCs sorting and Brownian motion bio-sensing technique. In 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010. 2010. p. 487-491. 5592441. (2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010). https://doi.org/10.1109/NEMS.2010.5592441