Existing e-learning system allows users to browse multimedia teaching materials, but only allows using text-based online assessment. Some study requires internship to learn processes of operations. Take medical education for example, interns need clinical observations of a variety of patients and learn how to diagnosis by the symptoms. Therefore, e-learning system should provide both of multimedia teaching material and multimedia assessment. The study proposes a new interactive-video e-learning system, and the experiment targets are medical students. The system provides case-based multimedia teaching materials. Instructor can online edit static text- and graphic-based teaching materials, as well as interactive multimedia video. Students can learn basic information and case histories from different cases. They are required to answer questions when watching documentary films. Simulated animation enables users to conduct operations in the virtual environment. Steps of operations can be recorded as personal portfolio of learning effect.