TY - GEN
T1 - An intergated microfluidic system for detecting human immunodeficiency virus in blood samples
AU - Chang, Wen Hsin
AU - Wang, Jung Hao
AU - Ling, Wei Shuo
AU - Cheng, Lie
AU - Wang, Chih Hung
AU - Wang, Shainn Wei
AU - Lee, Gwo Bin
PY - 2013
Y1 - 2013
N2 - To date, 25 million deaths have been caused by human immunodeficiency virus (HIV) infections. However, the lack of effective vaccines and drugs still leads to a high transmission rate of HIV. It was estimated that 2.7 million new infections occurred in 2011. Early and accurate diagnosis is essential to control the HIV transmission. Thus, many efforts have been made to explore a rapid and accurate method for HIV detection. The capabilities of antibody-based determination and nucleic-acid-based molecular diagnosis to detect HIV infections have been verified. Yet these methods have some intrinsic drawbacks. For example, antibody-based assays involve a lengthy process and may have an open-window issue while polymerase chain reaction (PCR)-based assays require delicate equipment and well-trained personnel. In this work, we present an integrated microfluidic system using a rolling circle amplification (RCA) assay which could automatically detect DNA fragment of HIV-1. With this approach, the HIV-1 could be detected automatically to reduce the risk of contamination with no human intervention. Additionally, the specially designed hook primer coated on the magnetic beads and a padlock probe both contribute to RNA extraction with higher specificity such that this developed system is capable of detecting HIV-1 from blood cells directly with improved accuracy. Successful demonstration for detection of HIV-1 DNA fragment has been achieved by using the proposed microfluidic platform.
AB - To date, 25 million deaths have been caused by human immunodeficiency virus (HIV) infections. However, the lack of effective vaccines and drugs still leads to a high transmission rate of HIV. It was estimated that 2.7 million new infections occurred in 2011. Early and accurate diagnosis is essential to control the HIV transmission. Thus, many efforts have been made to explore a rapid and accurate method for HIV detection. The capabilities of antibody-based determination and nucleic-acid-based molecular diagnosis to detect HIV infections have been verified. Yet these methods have some intrinsic drawbacks. For example, antibody-based assays involve a lengthy process and may have an open-window issue while polymerase chain reaction (PCR)-based assays require delicate equipment and well-trained personnel. In this work, we present an integrated microfluidic system using a rolling circle amplification (RCA) assay which could automatically detect DNA fragment of HIV-1. With this approach, the HIV-1 could be detected automatically to reduce the risk of contamination with no human intervention. Additionally, the specially designed hook primer coated on the magnetic beads and a padlock probe both contribute to RNA extraction with higher specificity such that this developed system is capable of detecting HIV-1 from blood cells directly with improved accuracy. Successful demonstration for detection of HIV-1 DNA fragment has been achieved by using the proposed microfluidic platform.
UR - http://www.scopus.com/inward/record.url?scp=84875465928&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84875465928&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2013.6474390
DO - 10.1109/MEMSYS.2013.6474390
M3 - Conference contribution
AN - SCOPUS:84875465928
SN - 9781467356558
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 903
EP - 906
BT - IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
T2 - IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Y2 - 20 January 2013 through 24 January 2013
ER -
