An intergated microfluidic system for detecting human immunodeficiency virus in blood samples

Wen Hsin Chang, Jung Hao Wang, Wei Shuo Ling, Lie Cheng, Chih Hung Wang, Shainn-Wei Wang, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To date, 25 million deaths have been caused by human immunodeficiency virus (HIV) infections. However, the lack of effective vaccines and drugs still leads to a high transmission rate of HIV. It was estimated that 2.7 million new infections occurred in 2011. Early and accurate diagnosis is essential to control the HIV transmission. Thus, many efforts have been made to explore a rapid and accurate method for HIV detection. The capabilities of antibody-based determination and nucleic-acid-based molecular diagnosis to detect HIV infections have been verified. Yet these methods have some intrinsic drawbacks. For example, antibody-based assays involve a lengthy process and may have an open-window issue while polymerase chain reaction (PCR)-based assays require delicate equipment and well-trained personnel. In this work, we present an integrated microfluidic system using a rolling circle amplification (RCA) assay which could automatically detect DNA fragment of HIV-1. With this approach, the HIV-1 could be detected automatically to reduce the risk of contamination with no human intervention. Additionally, the specially designed hook primer coated on the magnetic beads and a padlock probe both contribute to RNA extraction with higher specificity such that this developed system is capable of detecting HIV-1 from blood cells directly with improved accuracy. Successful demonstration for detection of HIV-1 DNA fragment has been achieved by using the proposed microfluidic platform.

Original languageEnglish
Title of host publicationIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Pages903-906
Number of pages4
DOIs
Publication statusPublished - 2013 Apr 2
EventIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan
Duration: 2013 Jan 202013 Jan 24

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

OtherIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
CountryTaiwan
CityTaipei
Period13-01-2013-01-24

Fingerprint

human immunodeficiency virus
Viruses
Microfluidics
blood
Blood
infectious diseases
Assays
antibodies
Antibodies
DNA
deoxyribonucleic acid
fragments
vaccines
polymerase chain reaction
primers
blood cells
Vaccines
hooks
Hooks
Polymerase chain reaction

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Chang, W. H., Wang, J. H., Ling, W. S., Cheng, L., Wang, C. H., Wang, S-W., & Lee, G. B. (2013). An intergated microfluidic system for detecting human immunodeficiency virus in blood samples. In IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 (pp. 903-906). [6474390] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2013.6474390
Chang, Wen Hsin ; Wang, Jung Hao ; Ling, Wei Shuo ; Cheng, Lie ; Wang, Chih Hung ; Wang, Shainn-Wei ; Lee, Gwo Bin. / An intergated microfluidic system for detecting human immunodeficiency virus in blood samples. IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013. 2013. pp. 903-906 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).
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title = "An intergated microfluidic system for detecting human immunodeficiency virus in blood samples",
abstract = "To date, 25 million deaths have been caused by human immunodeficiency virus (HIV) infections. However, the lack of effective vaccines and drugs still leads to a high transmission rate of HIV. It was estimated that 2.7 million new infections occurred in 2011. Early and accurate diagnosis is essential to control the HIV transmission. Thus, many efforts have been made to explore a rapid and accurate method for HIV detection. The capabilities of antibody-based determination and nucleic-acid-based molecular diagnosis to detect HIV infections have been verified. Yet these methods have some intrinsic drawbacks. For example, antibody-based assays involve a lengthy process and may have an open-window issue while polymerase chain reaction (PCR)-based assays require delicate equipment and well-trained personnel. In this work, we present an integrated microfluidic system using a rolling circle amplification (RCA) assay which could automatically detect DNA fragment of HIV-1. With this approach, the HIV-1 could be detected automatically to reduce the risk of contamination with no human intervention. Additionally, the specially designed hook primer coated on the magnetic beads and a padlock probe both contribute to RNA extraction with higher specificity such that this developed system is capable of detecting HIV-1 from blood cells directly with improved accuracy. Successful demonstration for detection of HIV-1 DNA fragment has been achieved by using the proposed microfluidic platform.",
author = "Chang, {Wen Hsin} and Wang, {Jung Hao} and Ling, {Wei Shuo} and Lie Cheng and Wang, {Chih Hung} and Shainn-Wei Wang and Lee, {Gwo Bin}",
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Chang, WH, Wang, JH, Ling, WS, Cheng, L, Wang, CH, Wang, S-W & Lee, GB 2013, An intergated microfluidic system for detecting human immunodeficiency virus in blood samples. in IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013., 6474390, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 903-906, IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013, Taipei, Taiwan, 13-01-20. https://doi.org/10.1109/MEMSYS.2013.6474390

An intergated microfluidic system for detecting human immunodeficiency virus in blood samples. / Chang, Wen Hsin; Wang, Jung Hao; Ling, Wei Shuo; Cheng, Lie; Wang, Chih Hung; Wang, Shainn-Wei; Lee, Gwo Bin.

IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013. 2013. p. 903-906 6474390 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Chang WH, Wang JH, Ling WS, Cheng L, Wang CH, Wang S-W et al. An intergated microfluidic system for detecting human immunodeficiency virus in blood samples. In IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013. 2013. p. 903-906. 6474390. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2013.6474390