TY - JOUR
T1 - An intermediate concentration of calcium with antioxidant supplement in culture medium enhances proliferation and decreases the aging of bone marrow mesenchymal stem cells
AU - Yang, Chung Da
AU - Chuang, Shu Chun
AU - Cheng, Tsung Lin
AU - Lee, Mon Juan
AU - Chen, Hui Ting
AU - Lin, Sung Yen
AU - Huang, Hsuan Ti
AU - Ho, Cheng Jung
AU - Lin, Yi Shan
AU - Kang, Lin
AU - Ho, Mei Ling
AU - Chang, Je Ken
AU - Chen, Chung Hwan
N1 - Funding Information:
Acknowledgments: We appreciate the technology support from Ching-Hua Yeh. We also appreciate the support from members of our Orthopedic Research Center and the Regenerative Medicine and Cell Therapy Research Center, Kaohsiung Medical University, and colleagues at Macromolecules Analysis lab, National Pingtung University of Science and Technology.
Funding Information:
Funding: This study was supported in part by the National Health Research Institute (NHRI-EX101-9935EI) of Taiwan, Kaohsiung Medical University Hospital (KMUH-107-7R54), Kaohsiung Municipal Ta-Tung Hospital (KMTTH-109-R014), Kaohsiung Medical University (KMU-TC109A02-1, NCTUKMU108-BIO-04, KMU-DK105009ĭKMU-DK 106003, KMU-DK(B) 110002 and KMTTH-DK(B) 110002-1), Kaohsiung Medical University-National Pingtung University of Science and Technology combined funding (NPUST-KMU-109-P002), and the Minister of Science and Technology of Taiwan (MOST 108-2314-B-037 -059 -MY3). The funders had no role in the study design, data collection, and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2020 Trans Tech Publications Ltd, Switzerland.
PY - 2021/2/2
Y1 - 2021/2/2
N2 - Human bone marrow stem cells (HBMSCs) are isolated from the bone marrow. Stem cells can self-renew and differentiate into various types of cells. They are able to regenerate kinds of tissue that are potentially used for tissue engineering. To maintain and expand these cells under culture conditions is difficult—they are easily triggered for differentiation or death. In this study, we describe a new culture formula to culture isolated HBMSCs. This new formula was modified from NCDB 153, a medium with low calcium, supplied with 5% FBS, extra growth factor added to it, and supplemented with N-acetyl-L-cysteine and L-ascorbic acid-2-phosphate to maintain the cells in a steady stage. The cells retain these characteristics as primarily isolated HBMSCs. Moreover, our new formula keeps HBMSCs with high proliferation rate and multiple linage differentiation ability, such as osteoblastogenesis, chondrogenesis, and adipogenesis. It also retains HBMSCs with stable chromosome, DNA, telomere length, and telomerase activity, even after long-term culture. Senescence can be minimized under this new formulation and carcinogenesis of stem cells can also be prevented. These modifications greatly enhance the survival rate, growth rate, and basal characteristics of isolated HBMSCs, which will be very helpful in stem cell research.
AB - Human bone marrow stem cells (HBMSCs) are isolated from the bone marrow. Stem cells can self-renew and differentiate into various types of cells. They are able to regenerate kinds of tissue that are potentially used for tissue engineering. To maintain and expand these cells under culture conditions is difficult—they are easily triggered for differentiation or death. In this study, we describe a new culture formula to culture isolated HBMSCs. This new formula was modified from NCDB 153, a medium with low calcium, supplied with 5% FBS, extra growth factor added to it, and supplemented with N-acetyl-L-cysteine and L-ascorbic acid-2-phosphate to maintain the cells in a steady stage. The cells retain these characteristics as primarily isolated HBMSCs. Moreover, our new formula keeps HBMSCs with high proliferation rate and multiple linage differentiation ability, such as osteoblastogenesis, chondrogenesis, and adipogenesis. It also retains HBMSCs with stable chromosome, DNA, telomere length, and telomerase activity, even after long-term culture. Senescence can be minimized under this new formulation and carcinogenesis of stem cells can also be prevented. These modifications greatly enhance the survival rate, growth rate, and basal characteristics of isolated HBMSCs, which will be very helpful in stem cell research.
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U2 - 10.3390/ijms22042095
DO - 10.3390/ijms22042095
M3 - Article
C2 - 33672524
AN - SCOPUS:85101199188
VL - 22
SP - 1
EP - 18
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 4
M1 - 2095
ER -