TY - GEN
T1 - An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids
AU - Chang, Wen Hsin
AU - Yang, Sung Yi
AU - Wang, Chih Hung
AU - Li, Ping Chen
AU - Jan, Fuh Jyh
AU - Chen, Tzong-Yueh
AU - Lee, Gwo Bin
PY - 2012/6/1
Y1 - 2012/6/1
N2 - Early detection of pathogens is important for surveillance and control of infectious diseases among Phalaenopsis orchids. Therefore, the current study presents an integrated microfluidic system for rapid and automatic detection of Phalaenopsis orchid viruses. The entire process, including pathogen-specific ribonucleic acid (RNA) purification, nucleic acid amplification using reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) and optical detection by measuring turbidity change, can be automatically performed on a single chip within 65 minutes. This is the first time that an integrated microfluidic system for the detection of Phalaenopsis orchid viruses has been demonstrated. The specificity and sensitivity of the system were also explored in this study to validate its performance. One of the most prevalent orchid viruses, Cymbidium mosaic virus (CymMV), was used in current study to demonstrate the capabilities of the developed system.
AB - Early detection of pathogens is important for surveillance and control of infectious diseases among Phalaenopsis orchids. Therefore, the current study presents an integrated microfluidic system for rapid and automatic detection of Phalaenopsis orchid viruses. The entire process, including pathogen-specific ribonucleic acid (RNA) purification, nucleic acid amplification using reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) and optical detection by measuring turbidity change, can be automatically performed on a single chip within 65 minutes. This is the first time that an integrated microfluidic system for the detection of Phalaenopsis orchid viruses has been demonstrated. The specificity and sensitivity of the system were also explored in this study to validate its performance. One of the most prevalent orchid viruses, Cymbidium mosaic virus (CymMV), was used in current study to demonstrate the capabilities of the developed system.
UR - http://www.scopus.com/inward/record.url?scp=84861542459&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84861542459&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2012.6196757
DO - 10.1109/NEMS.2012.6196757
M3 - Conference contribution
AN - SCOPUS:84861542459
SN - 9781467311243
T3 - 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
SP - 204
EP - 207
BT - 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
T2 - 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
Y2 - 5 March 2012 through 8 March 2012
ER -
