An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids

Wen Hsin Chang, Sung Yi Yang, Chih Hung Wang, Ping Chen Li, Fuh Jyh Jan, Tzong-Yueh Chen, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Early detection of pathogens is important for surveillance and control of infectious diseases among Phalaenopsis orchids. Therefore, the current study presents an integrated microfluidic system for rapid and automatic detection of Phalaenopsis orchid viruses. The entire process, including pathogen-specific ribonucleic acid (RNA) purification, nucleic acid amplification using reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) and optical detection by measuring turbidity change, can be automatically performed on a single chip within 65 minutes. This is the first time that an integrated microfluidic system for the detection of Phalaenopsis orchid viruses has been demonstrated. The specificity and sensitivity of the system were also explored in this study to validate its performance. One of the most prevalent orchid viruses, Cymbidium mosaic virus (CymMV), was used in current study to demonstrate the capabilities of the developed system.

Original languageEnglish
Title of host publication2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
Pages204-207
Number of pages4
DOIs
Publication statusPublished - 2012 Jun 1
Event7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 - Kyoto, Japan
Duration: 2012 Mar 52012 Mar 8

Publication series

Name2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012

Other

Other7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
CountryJapan
CityKyoto
Period12-03-0512-03-08

Fingerprint

Viruses
Amplification
Pathogens
Microfluidics
Nucleic acids
Turbidity
Transcription
RNA
Purification

All Science Journal Classification (ASJC) codes

  • Engineering (miscellaneous)

Cite this

Chang, W. H., Yang, S. Y., Wang, C. H., Li, P. C., Jan, F. J., Chen, T-Y., & Lee, G. B. (2012). An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids. In 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 (pp. 204-207). [6196757] (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012). https://doi.org/10.1109/NEMS.2012.6196757
Chang, Wen Hsin ; Yang, Sung Yi ; Wang, Chih Hung ; Li, Ping Chen ; Jan, Fuh Jyh ; Chen, Tzong-Yueh ; Lee, Gwo Bin. / An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids. 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012. 2012. pp. 204-207 (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012).
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abstract = "Early detection of pathogens is important for surveillance and control of infectious diseases among Phalaenopsis orchids. Therefore, the current study presents an integrated microfluidic system for rapid and automatic detection of Phalaenopsis orchid viruses. The entire process, including pathogen-specific ribonucleic acid (RNA) purification, nucleic acid amplification using reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) and optical detection by measuring turbidity change, can be automatically performed on a single chip within 65 minutes. This is the first time that an integrated microfluidic system for the detection of Phalaenopsis orchid viruses has been demonstrated. The specificity and sensitivity of the system were also explored in this study to validate its performance. One of the most prevalent orchid viruses, Cymbidium mosaic virus (CymMV), was used in current study to demonstrate the capabilities of the developed system.",
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Chang, WH, Yang, SY, Wang, CH, Li, PC, Jan, FJ, Chen, T-Y & Lee, GB 2012, An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids. in 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012., 6196757, 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012, pp. 204-207, 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012, Kyoto, Japan, 12-03-05. https://doi.org/10.1109/NEMS.2012.6196757

An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids. / Chang, Wen Hsin; Yang, Sung Yi; Wang, Chih Hung; Li, Ping Chen; Jan, Fuh Jyh; Chen, Tzong-Yueh; Lee, Gwo Bin.

2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012. 2012. p. 204-207 6196757 (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Chang WH, Yang SY, Wang CH, Li PC, Jan FJ, Chen T-Y et al. An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids. In 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012. 2012. p. 204-207. 6196757. (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012). https://doi.org/10.1109/NEMS.2012.6196757