An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids

Wen Hsin Chang; Sung Yi Yang; Chih Hung Wang; Ping Chen Li; Fuh Jyh Jan; Tzong-Yueh Chen; Gwo Bin Lee

doi:10.1109/NEMS.2012.6196757

An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids

Wen Hsin Chang, Sung Yi Yang, Chih Hung Wang, Ping Chen Li, Fuh Jyh Jan, Tzong-Yueh Chen, Gwo Bin Lee

Department of Biotechnology and Bioindustry Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

Early detection of pathogens is important for surveillance and control of infectious diseases among Phalaenopsis orchids. Therefore, the current study presents an integrated microfluidic system for rapid and automatic detection of Phalaenopsis orchid viruses. The entire process, including pathogen-specific ribonucleic acid (RNA) purification, nucleic acid amplification using reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) and optical detection by measuring turbidity change, can be automatically performed on a single chip within 65 minutes. This is the first time that an integrated microfluidic system for the detection of Phalaenopsis orchid viruses has been demonstrated. The specificity and sensitivity of the system were also explored in this study to validate its performance. One of the most prevalent orchid viruses, Cymbidium mosaic virus (CymMV), was used in current study to demonstrate the capabilities of the developed system.

Original language	English
Title of host publication	2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
Pages	204-207
Number of pages	4
DOIs	https://doi.org/10.1109/NEMS.2012.6196757
Publication status	Published - 2012 Jun 1
Event	7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 - Kyoto, Japan Duration: 2012 Mar 5 → 2012 Mar 8

Publication series

Name	2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012

Other

Other	7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
Country	Japan
City	Kyoto
Period	12-03-05 → 12-03-08

All Science Journal Classification (ASJC) codes

Engineering (miscellaneous)

Access to Document

10.1109/NEMS.2012.6196757

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Chang, W. H., Yang, S. Y., Wang, C. H., Li, P. C., Jan, F. J., Chen, T-Y., & Lee, G. B. (2012). An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids. In 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 (pp. 204-207). [6196757] (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012). https://doi.org/10.1109/NEMS.2012.6196757

Chang, Wen Hsin ; Yang, Sung Yi ; Wang, Chih Hung ; Li, Ping Chen ; Jan, Fuh Jyh ; Chen, Tzong-Yueh ; Lee, Gwo Bin. / An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids. 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012. 2012. pp. 204-207 (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012).

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title = "An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids",

abstract = "Early detection of pathogens is important for surveillance and control of infectious diseases among Phalaenopsis orchids. Therefore, the current study presents an integrated microfluidic system for rapid and automatic detection of Phalaenopsis orchid viruses. The entire process, including pathogen-specific ribonucleic acid (RNA) purification, nucleic acid amplification using reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) and optical detection by measuring turbidity change, can be automatically performed on a single chip within 65 minutes. This is the first time that an integrated microfluidic system for the detection of Phalaenopsis orchid viruses has been demonstrated. The specificity and sensitivity of the system were also explored in this study to validate its performance. One of the most prevalent orchid viruses, Cymbidium mosaic virus (CymMV), was used in current study to demonstrate the capabilities of the developed system.",

author = "Chang, {Wen Hsin} and Yang, {Sung Yi} and Wang, {Chih Hung} and Li, {Ping Chen} and Jan, {Fuh Jyh} and Tzong-Yueh Chen and Lee, {Gwo Bin}",

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Chang, WH, Yang, SY, Wang, CH, Li, PC, Jan, FJ, Chen, T-Y & Lee, GB 2012, An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids. in 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012., 6196757, 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012, pp. 204-207, 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012, Kyoto, Japan, 12-03-05. https://doi.org/10.1109/NEMS.2012.6196757

An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids. / Chang, Wen Hsin; Yang, Sung Yi; Wang, Chih Hung; Li, Ping Chen; Jan, Fuh Jyh; Chen, Tzong-Yueh; Lee, Gwo Bin.

2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012. 2012. p. 204-207 6196757 (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - Chen, Tzong-Yueh

AU - Lee, Gwo Bin

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AB - Early detection of pathogens is important for surveillance and control of infectious diseases among Phalaenopsis orchids. Therefore, the current study presents an integrated microfluidic system for rapid and automatic detection of Phalaenopsis orchid viruses. The entire process, including pathogen-specific ribonucleic acid (RNA) purification, nucleic acid amplification using reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) and optical detection by measuring turbidity change, can be automatically performed on a single chip within 65 minutes. This is the first time that an integrated microfluidic system for the detection of Phalaenopsis orchid viruses has been demonstrated. The specificity and sensitivity of the system were also explored in this study to validate its performance. One of the most prevalent orchid viruses, Cymbidium mosaic virus (CymMV), was used in current study to demonstrate the capabilities of the developed system.

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Chang WH, Yang SY, Wang CH, Li PC, Jan FJ, Chen T-Y et al. An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids. In 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012. 2012. p. 204-207. 6196757. (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012). https://doi.org/10.1109/NEMS.2012.6196757