An optical-induced platform for multiple genes transfection

Hsin Tzu Kuo, You Hsun Lee, Chih Hung Wang, Chen-Min Chang, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Gene transfection is an important technology in biological applications. Electroporation is one of gene transfection methods that deliver extracellular genetic materials into cells by using a high electrical field to forming pores on cell surface. However, the high voltage provided by an electroporator may impair cells to survive and cause low efficiency of yield rate. In this study, a new platform was developed for gene transfection under a lower applied voltage by utilizing an optical induced non-uniform electric field. To obtain higher transfection efficiency, a multi-spot optical image was projected to the gene transfection chip, resulting in localized non-uniform electric fields generated from these optical patterns. The experimental results in this study showed that one or more plasmid carried fluorescence genes could be effectively transfected into mammalian cells and the cells successfully expressed foreign proteins by using the developed optical-induced gene transfection platform. Moreover, the transfection efficiency of optical-induced gene transfection platform was significantly higher than the traditional electroporation technology. This developed platform may provide a simpler and more efficient tool for gene transfection.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1657-1659
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12-10-2812-11-01

Fingerprint

Genes
Electric fields
Electric potential
Plasmids
Fluorescence
Cells
Proteins

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Kuo, H. T., Lee, Y. H., Wang, C. H., Chang, C-M., & Lee, G. B. (2012). An optical-induced platform for multiple genes transfection. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1657-1659). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.
Kuo, Hsin Tzu ; Lee, You Hsun ; Wang, Chih Hung ; Chang, Chen-Min ; Lee, Gwo Bin. / An optical-induced platform for multiple genes transfection. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 1657-1659 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).
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Kuo, HT, Lee, YH, Wang, CH, Chang, C-M & Lee, GB 2012, An optical-induced platform for multiple genes transfection. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Chemical and Biological Microsystems Society, pp. 1657-1659, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 12-10-28.

An optical-induced platform for multiple genes transfection. / Kuo, Hsin Tzu; Lee, You Hsun; Wang, Chih Hung; Chang, Chen-Min; Lee, Gwo Bin.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1657-1659 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Kuo HT, Lee YH, Wang CH, Chang C-M, Lee GB. An optical-induced platform for multiple genes transfection. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 1657-1659. (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).