An optical measurement using the DNA hybridization method for specific species of bacteria detection

Chia Hsien Yeh, Yu Huai Chang, Hong-Ping Lin, Tsung Chain Chang, Yu-Cheng Lin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports a developed DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, and a flatbed scanner to detect the hybridization signal. There was a significant difference in grayscale between the experimental samples and the negative control after 18 min. The detection limit for a DNA sample is 8.25 ng/mL with 5 μM of probe and 10-3 μM of AuNPs-streptavidin. The probe specific tests for different DNA samples were discussed: (1) the probe specific tests for various species of bacteria, and (2) the probe specific tests between the various strains of a single species and various species of a single genus. The developed DNA hybridization method is easy, convenient, and rapid.

Original languageEnglish
Title of host publicationIEEE Sensors 2010 Conference, SENSORS 2010
Pages474-476
Number of pages3
DOIs
Publication statusPublished - 2010 Dec 1
Event9th IEEE Sensors Conference 2010, SENSORS 2010 - Waikoloa, HI, United States
Duration: 2010 Nov 12010 Nov 4

Publication series

NameProceedings of IEEE Sensors

Other

Other9th IEEE Sensors Conference 2010, SENSORS 2010
CountryUnited States
CityWaikoloa, HI
Period10-11-0110-11-04

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

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