An optical measurement using the DNA hybridization method for specific species of bacteria detection

Chia Hsien Yeh; Yu Huai Chang; Hong Ping Lin; Tsung Chain Chang; Yu Cheng Lin

doi:10.1109/ICSENS.2010.5690160

An optical measurement using the DNA hybridization method for specific species of bacteria detection

Chia Hsien Yeh, Yu Huai Chang, Hong Ping Lin, Tsung Chain Chang, Yu Cheng Lin

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This paper reports a developed DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, and a flatbed scanner to detect the hybridization signal. There was a significant difference in grayscale between the experimental samples and the negative control after 18 min. The detection limit for a DNA sample is 8.25 ng/mL with 5 μM of probe and 10^-3 μM of AuNPs-streptavidin. The probe specific tests for different DNA samples were discussed: (1) the probe specific tests for various species of bacteria, and (2) the probe specific tests between the various strains of a single species and various species of a single genus. The developed DNA hybridization method is easy, convenient, and rapid.

Original language	English
Title of host publication	IEEE Sensors 2010 Conference, SENSORS 2010
Pages	474-476
Number of pages	3
DOIs	https://doi.org/10.1109/ICSENS.2010.5690160
Publication status	Published - 2010
Event	9th IEEE Sensors Conference 2010, SENSORS 2010 - Waikoloa, HI, United States Duration: 2010 Nov 1 → 2010 Nov 4

Publication series

Name	Proceedings of IEEE Sensors

Other

Other	9th IEEE Sensors Conference 2010, SENSORS 2010
Country/Territory	United States
City	Waikoloa, HI
Period	10-11-01 → 10-11-04

All Science Journal Classification (ASJC) codes

Electrical and Electronic Engineering

Access to Document

10.1109/ICSENS.2010.5690160

Cite this

@inproceedings{69b9835451a84362854719a7f1f966e8,

title = "An optical measurement using the DNA hybridization method for specific species of bacteria detection",

abstract = "This paper reports a developed DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, and a flatbed scanner to detect the hybridization signal. There was a significant difference in grayscale between the experimental samples and the negative control after 18 min. The detection limit for a DNA sample is 8.25 ng/mL with 5 μM of probe and 10-3 μM of AuNPs-streptavidin. The probe specific tests for different DNA samples were discussed: (1) the probe specific tests for various species of bacteria, and (2) the probe specific tests between the various strains of a single species and various species of a single genus. The developed DNA hybridization method is easy, convenient, and rapid.",

author = "Yeh, {Chia Hsien} and Chang, {Yu Huai} and Lin, {Hong Ping} and Chang, {Tsung Chain} and Lin, {Yu Cheng}",

year = "2010",

doi = "10.1109/ICSENS.2010.5690160",

language = "English",

isbn = "9781424481682",

series = "Proceedings of IEEE Sensors",

pages = "474--476",

booktitle = "IEEE Sensors 2010 Conference, SENSORS 2010",

note = "9th IEEE Sensors Conference 2010, SENSORS 2010 ; Conference date: 01-11-2010 Through 04-11-2010",

}

Yeh, CH, Chang, YH, Lin, HP, Chang, TC & Lin, YC 2010, An optical measurement using the DNA hybridization method for specific species of bacteria detection. in IEEE Sensors 2010 Conference, SENSORS 2010., 5690160, Proceedings of IEEE Sensors, pp. 474-476, 9th IEEE Sensors Conference 2010, SENSORS 2010, Waikoloa, HI, United States, 10-11-01. https://doi.org/10.1109/ICSENS.2010.5690160

TY - GEN

T1 - An optical measurement using the DNA hybridization method for specific species of bacteria detection

AU - Yeh, Chia Hsien

AU - Chang, Yu Huai

AU - Lin, Hong Ping

AU - Chang, Tsung Chain

AU - Lin, Yu Cheng

PY - 2010

Y1 - 2010

N2 - This paper reports a developed DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, and a flatbed scanner to detect the hybridization signal. There was a significant difference in grayscale between the experimental samples and the negative control after 18 min. The detection limit for a DNA sample is 8.25 ng/mL with 5 μM of probe and 10-3 μM of AuNPs-streptavidin. The probe specific tests for different DNA samples were discussed: (1) the probe specific tests for various species of bacteria, and (2) the probe specific tests between the various strains of a single species and various species of a single genus. The developed DNA hybridization method is easy, convenient, and rapid.

AB - This paper reports a developed DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, and a flatbed scanner to detect the hybridization signal. There was a significant difference in grayscale between the experimental samples and the negative control after 18 min. The detection limit for a DNA sample is 8.25 ng/mL with 5 μM of probe and 10-3 μM of AuNPs-streptavidin. The probe specific tests for different DNA samples were discussed: (1) the probe specific tests for various species of bacteria, and (2) the probe specific tests between the various strains of a single species and various species of a single genus. The developed DNA hybridization method is easy, convenient, and rapid.

UR - http://www.scopus.com/inward/record.url?scp=79951940165&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79951940165&partnerID=8YFLogxK

U2 - 10.1109/ICSENS.2010.5690160

DO - 10.1109/ICSENS.2010.5690160

M3 - Conference contribution

AN - SCOPUS:79951940165

SN - 9781424481682

T3 - Proceedings of IEEE Sensors

SP - 474

EP - 476

BT - IEEE Sensors 2010 Conference, SENSORS 2010

T2 - 9th IEEE Sensors Conference 2010, SENSORS 2010

Y2 - 1 November 2010 through 4 November 2010

ER -

An optical measurement using the DNA hybridization method for specific species of bacteria detection

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