An optoelectrokinetic technique for programmable particle manipulation and bead-based biosignal enhancement

Kuan Chih Wang, Aloke Kumar, Stuart J. Williams, Nicolas G. Green, Kyung Chun Kim, Han Sheng Chuang

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Technologies that can enable concentration of low-abundance biomarkers are essential for early diagnosis of diseases. In this study, an optoelectrokinetic technique, termed Rapid Electrokinetic Patterning (REP), was used to enable dynamic particle manipulation in bead-based bioassays. Various manipulation capabilities, such as micro/nanoparticle aggregation, translation, sorting and patterning, were developed. The technique allows for versatile multi-parameter (voltage, light intensity and frequency) based modulation and dynamically addressable manipulation with simple device fabrication. Signal enhancement of a bead-based bioassay was demonstrated using dilute biotin-fluorescein isothiocyanate (FITC) solutions mixed with streptavidin-conjugated particles and rapidly concentrated with the technique. As compared with a conventional ELISA reader, the REP-enabled detection achieved a minimal readout of 3.87 nM, which was a 100-fold improvement in sensitivity. The multi-functional platform provides an effective measure to enhance detection levels in more bead-based bioassays.

Original languageEnglish
Pages (from-to)3958-3967
Number of pages10
JournalLab on a Chip
Volume14
Issue number20
DOIs
Publication statusPublished - 2014 Oct 21

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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