An oral nervous necrosis virus vaccine using Vibrio anguillarum as an expression host provides early protection

Yi Min Chen, Ching Hung Shih, Hsiao Chien Liu, Cheng Lung Wu, Chin Chiu Lin, Han Ching Wang, Tzong Yueh Chen, Huey Lang Yang, John Han You Lin

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Oral vaccination is the most desirable immunization method by which to prevent fish disease occurring at the early larval stage. We developed an Escherichia coli-based oral nervous necrosis virus (NNV) subunit vaccine and demonstrated its effectiveness in orange spotted grouper (Epinephelus coioides) fry. However, the incubation period was determined to be over 2. weeks. To enhance the efficacy and shorten the incubation period, a safe and digestible biological adjuvant should be found. Vibrio anguillarum, a common marine bacterium with immune-stimulatory capability was selected as an expression host. An expression vector containing its heat shock protein 60 gene (groE) as an inducible promoter was constructed to express recombinant NNV coat protein as an antigen. The recombinant V. anguillarum was inactivated and encapsulated in Artemia nauplii to formulate a V. anguillarum-based oral vaccine. Compared to the E. coli-based oral vaccine, this novel oral vaccine not only elicited a higher expression of Mx (encoding for an anti-viral protein) in the brain and viscera but also a higher specific antibody titer against NNV in the first week after inoculation. Meanwhile, a higher survival rate compared to an equal dosage of an E. coli-based oral vaccine was also noticed in two individual challenge trials, indicating that an antigen expressed in an V. anguillarum-based oral vaccine could enhance efficacy in a shorter incubation period and could reduce the risk of NNV infection.

Original languageEnglish
Pages (from-to)26-33
Number of pages8
Issue number1-2
Publication statusPublished - 2011 Nov 16

All Science Journal Classification (ASJC) codes

  • Aquatic Science


Dive into the research topics of 'An oral nervous necrosis virus vaccine using Vibrio anguillarum as an expression host provides early protection'. Together they form a unique fingerprint.

Cite this