An ultrasonic tool for nerve conduction block in diabetic rat models

Yee Fun Lee, Chou-Ching Lin, Jung Sung Cheng, Gin Shin Chen

Research output: Contribution to journalArticle

Abstract

Nerve conduction block with a high intensity-focused ultrasound (HIFU) transducer has been performed in normal and diabetic animal models recently. HIFU can reversibly block the conduction of peripheral nerves without damaging the nerves while using an appropriate ultrasonic parameter. Temporary and partial block of the action potentials of nerves shows that HIFU has the potential to be a useful clinical treatment for pain relief. This work demonstrates the procedures for suppressing the action potentials of neuropathic nerves in diabetic rats in vivo using an HIFU transducer. The first step is to generate adult male diabetic neuropathic rats by streptozotocin (STZ) injection. The second step is to evaluate the peripheral diabetic neuropathy in STZ-induced diabetic rats by an electronic von Frey probe and a hot plate. The final step is to record in vivo extracellular action potentials of the nerve exposed to HIFU sonication. The method showed here may benefit the study of ultrasound analgesic applications.

Original languageEnglish
Article numbere55675
JournalJournal of Visualized Experiments
Volume2017
Issue number128
DOIs
Publication statusPublished - 2017 Oct 20

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Nerve Block
Neural Conduction
Ultrasonics
Action Potentials
Rats
Streptozocin
Transducers
Sonication
Diabetic Neuropathies
Peripheral Nervous System Diseases
Peripheral Nerves
Analgesics
Animal Models
Pain
Injections
Animals
Therapeutics

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Lee, Yee Fun ; Lin, Chou-Ching ; Cheng, Jung Sung ; Chen, Gin Shin. / An ultrasonic tool for nerve conduction block in diabetic rat models. In: Journal of Visualized Experiments. 2017 ; Vol. 2017, No. 128.
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An ultrasonic tool for nerve conduction block in diabetic rat models. / Lee, Yee Fun; Lin, Chou-Ching; Cheng, Jung Sung; Chen, Gin Shin.

In: Journal of Visualized Experiments, Vol. 2017, No. 128, e55675, 20.10.2017.

Research output: Contribution to journalArticle

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