Analysis of aristolochic acid in nine sources of Xixin, a traditional Chinese medicine, by liquid chromatography/atmospheric pressure chemical ionization/tandem mass spectrometry

Ting Ting Jong, Maw Rong Lee, Shun Sheng Hsiao, Jar Lung Hsai, Tian Shung Wu, Shu Tuan Chiang, Shao Qing Cai

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71 Citations (Scopus)

Abstract

Aristolochic acid I (AA-I), which is a known nephrotoxin, is found in a commonly used Chinese medicine, Xixin, that originates from nine Asarum species (Aristolochiaceae) found in China. A method has been developed using reversed-phase liquid chromatography coupled with atmospheric pressure chemical ionization (APCI) tandem mass spectrometry under the positive ion detection mode [LC/(+)APCI/MS/MS] to determine the amount of AA-I in Xixin. The limit of detection of AA-I, estimated by monitoring with LC/MS/MS, was at the low μg/ l level. By applying this method to methanol extracts of nine Asarum species, the concentrations of AA-I were found to range from 3.3 ng/mg (Asarum sieboldii) to 3376.9 ng/mg (Asarum crispulatum).

Original languageEnglish
Pages (from-to)831-837
Number of pages7
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume33
Issue number4
DOIs
Publication statusPublished - 2003 Nov 24

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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