Analysis of early embryonic great-vessel microcirculation in zebrafish using high-speed confocal μPIV

Chia-Yuan Chen, Michael J. Patrick, Paola Corti, William Kowalski, Beth L. Roman, Kerem Pekkan

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In the developing cardiovascular system, hemodynamic vascular loading is critical for angiogenesis and cardiovascular adaptation. Normal zebrafish embryos with transgenically-labeled endothelial and red blood cells provide an excellent in vivo model for studying the fluid-flow induced vascular loading. To characterize the developmental hemodynamics of early embryonic great-vessel microcirculation in the zebrafish embryo, two complementary studies (experimental and numerical) are presented. Quantitative comparison of the wall shear stress (WSS) at the first aortic arch (AA1) of wild-type zebrafish embryos during two consecutive developmental stages is presented, using time-resolved confocal micro-particle image velocimetry (μPIV). Analysis showed that there was significant WSS difference between 32 and 48 h post-fertilization (hpf) wild-type embryos, which correlates with normal arch morphogenesis. The vascular distensibility of the arch wall at systole and the acceleration/deceleration rates of time-lapse phase-averaged streamwise blood flow curves were also analyzed. To estimate the influence of a novel intermittent red-blood cell (RBC) loading on the endothelium, a numerical two-phase, volume of fluid (VOF) flow model was further developed with realistic in vivo conditions. These studies showed that near-wall effects and cell clustering increased WSS augmentation at a minimum of 15% when the distance of RBC from arch vessel wall was less than 3 μm or when RBC cell-to-cell distance was less than 3 μm. When compared to a smooth wall, the WSS augmentation increased by a factor of ~1.4 due to the roughness of the wall created by the endothelial cell profile. These results quantitatively highlight the contribution of individual RBC flow patterns on endothelial WSS in great-vessel microcirculation and will benefit the quantitative understanding of mechanotransduction in embryonic great vessel biology, including arteriovenous malformations (AVM).

Original languageEnglish
Pages (from-to)305-321
Number of pages17
JournalBiorheology
Volume48
Issue number5-6
DOIs
Publication statusPublished - 2011 Dec 1

Fingerprint

Rheology
Zebrafish
Microcirculation
Erythrocytes
Embryonic Structures
Blood Vessels
Hemodynamics
Deceleration
Systole
Arteriovenous Malformations
Cardiovascular System
Thoracic Aorta
Morphogenesis
Fertilization
Cell Wall
Endothelium
Cluster Analysis
Endothelial Cells

All Science Journal Classification (ASJC) codes

  • Physiology (medical)
  • Physiology

Cite this

Chen, Chia-Yuan ; Patrick, Michael J. ; Corti, Paola ; Kowalski, William ; Roman, Beth L. ; Pekkan, Kerem. / Analysis of early embryonic great-vessel microcirculation in zebrafish using high-speed confocal μPIV. In: Biorheology. 2011 ; Vol. 48, No. 5-6. pp. 305-321.
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Analysis of early embryonic great-vessel microcirculation in zebrafish using high-speed confocal μPIV. / Chen, Chia-Yuan; Patrick, Michael J.; Corti, Paola; Kowalski, William; Roman, Beth L.; Pekkan, Kerem.

In: Biorheology, Vol. 48, No. 5-6, 01.12.2011, p. 305-321.

Research output: Contribution to journalArticle

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AU - Patrick, Michael J.

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