Analysis of global methylation using a Zta-expressing nasopharyngeal carcinoma cell line

Ying Fan Chen, Chao Ling Tung, Yao Chang, Wen Chi Hsiao, lh Jen Su, H. Sunny Sun

Research output: Contribution to journalArticlepeer-review

Abstract

EBV infects more than 90% of the human population and persists in most individuals as a latent infection where the viral genome is silenced by host-driven methylation. The lytic cycle is initiated when the viral protein Zta binds to methylated BRLF1 and BRRF1 promoters. Although studies reveal the role of Zta and methylation changes in the viral genome upon EBV infection to reactivation, whether Zta plays any role in alteration of methylation in the host genome remains unknown. Using an inducible model, we demonstrate that global DNA methylation, based on whole-genome 5-methylcytosine content, and regional DNA methylation in repetitive elements, imprinting genes and the X chromosome, remains unchanged in response to Zta expression. Expression of DNA methyltransferases was also unaffected by ectopically expressed Zta. Our data imply that alteration of host gene expression following EBV reactivation may reflect methylation-independent Zta-mediated gene activation and not epigenetic modification of the host genome.

Original languageEnglish
Pages (from-to)205-213
Number of pages9
JournalGenomics
Volume97
Issue number4
DOIs
Publication statusPublished - 2011 Apr

All Science Journal Classification (ASJC) codes

  • Genetics

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