Analysis of interferon gamma protein expression in zebrafish (Danio rerio)

Sohye Yoon, Ayham Alnabulsi, Ting Yu Wang, Po Tsang Lee, Tzong Yueh Chen, Steve Bird, Jun Zou, Christopher J. Secombes

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

IFN-γ is a major effector cytokine, produced to induce type I immune responses. It has been cloned in several fish species including zebrafish, however to date few studies have looked at IFN-γ protein expression and bioactivity in fish. Hence, the current study focused on developing a monoclonal antibody (moAb) against zfIFN-γ. We show that the zfIFN-γ moAb specifically recognises E. coli produced recombinant IFN-γ protein and zfIFN-γ produced in transfected HEK293 cells, by Western blot analysis. Next we analysed the production of the native protein following expression induced by PHA stimulation of leukocytes in vitro or antigen re-stimulation in vivo. We show the IFN-γ protein is produced as a dimer, and that a good correlation exists between transcript expression levels and protein levels.

Original languageEnglish
Pages (from-to)79-86
Number of pages8
JournalFish and Shellfish Immunology
Volume57
DOIs
Publication statusPublished - 2016 Oct 1

Fingerprint

interferon-gamma
Danio rerio
Interferon-gamma
protein synthesis
protein
monoclonal antibodies
Fish
Proteins
proteins
Monoclonal Antibodies
antibody
fish
Recombinant proteins
leukocytes
Western blotting
cytokines
Bioactivity
bioactivity
immune response
Dimers

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

Cite this

Yoon, Sohye ; Alnabulsi, Ayham ; Wang, Ting Yu ; Lee, Po Tsang ; Chen, Tzong Yueh ; Bird, Steve ; Zou, Jun ; Secombes, Christopher J. / Analysis of interferon gamma protein expression in zebrafish (Danio rerio). In: Fish and Shellfish Immunology. 2016 ; Vol. 57. pp. 79-86.
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Yoon, S, Alnabulsi, A, Wang, TY, Lee, PT, Chen, TY, Bird, S, Zou, J & Secombes, CJ 2016, 'Analysis of interferon gamma protein expression in zebrafish (Danio rerio)', Fish and Shellfish Immunology, vol. 57, pp. 79-86. https://doi.org/10.1016/j.fsi.2016.08.023

Analysis of interferon gamma protein expression in zebrafish (Danio rerio). / Yoon, Sohye; Alnabulsi, Ayham; Wang, Ting Yu; Lee, Po Tsang; Chen, Tzong Yueh; Bird, Steve; Zou, Jun; Secombes, Christopher J.

In: Fish and Shellfish Immunology, Vol. 57, 01.10.2016, p. 79-86.

Research output: Contribution to journalArticle

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