Analysis of interferon gamma protein expression in zebrafish (Danio rerio)

Sohye Yoon, Ayham Alnabulsi, Ting Yu Wang, Po Tsang Lee, Tzong Yueh Chen, Steve Bird, Jun Zou, Christopher J. Secombes

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

IFN-γ is a major effector cytokine, produced to induce type I immune responses. It has been cloned in several fish species including zebrafish, however to date few studies have looked at IFN-γ protein expression and bioactivity in fish. Hence, the current study focused on developing a monoclonal antibody (moAb) against zfIFN-γ. We show that the zfIFN-γ moAb specifically recognises E. coli produced recombinant IFN-γ protein and zfIFN-γ produced in transfected HEK293 cells, by Western blot analysis. Next we analysed the production of the native protein following expression induced by PHA stimulation of leukocytes in vitro or antigen re-stimulation in vivo. We show the IFN-γ protein is produced as a dimer, and that a good correlation exists between transcript expression levels and protein levels.

Original languageEnglish
Pages (from-to)79-86
Number of pages8
JournalFish and Shellfish Immunology
Volume57
DOIs
Publication statusPublished - 2016 Oct 1

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

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