Analysis of sugar phosphates in plants by ion chromatography on a titanium dioxide column with pulsed amperometric detection

Yoko Sekiguchi, Naoto Mitsuhashi, Yoshinori Inoue, Hitoshi Yagisawa, Tetsuro Mimura

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

This paper describes the development of a practical method for the analysis of sugar phosphates from the model higher plant Arabidopsis thaliana by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The extraction method of sugar phosphates from higher plants was first optimized for HPAEC-PAD analysis. In order to improve the resolution in HPAEC-PAD, a column packed with titanium dioxide resin was used. The titanium dioxide column was used as a trap-column for sugar phosphates and nucleotides, for the removal of sample matrices. Sample pretreatment was achieved in-line and automatically using a six-port valve placed after the injection valve.

Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalJournal of Chromatography A
Volume1039
Issue number1-2
DOIs
Publication statusPublished - 2004 Jun 11

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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