Analysis of the expression of Fas-L in nasopharyngeal carcinoma tissues

Sen-Tien Tsai, Sheen-Yie Fang, Yin Tai Jin, Ih Jen Su, Bei-Chang Yang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Nasopharyngeal carcinoma (NPC) is an epithelial cancer with a high incidence in Southeast Asia. How it escapes attack from the host immune system is not fully understood. Recently, pieces of evidence show that Fas- ligand (Fas-L)-mediated apoptosis may be involved in immune privilege of tumours. To determine whether a similar mechanism may exist in NPC, the expression of Fas-L was analysed. Biopsy specimens of the nasopharynx were taken from 27 NPC patients. Histologically, they were either non-keratinizing or undifferentiated carcinomas. Nasopharyngeal biopsies of 11 other patients that proved to have no tumour served as control. The transcripts of Fas-L were detected by reverse transcription-polymerase chain reaction. Localization of Fas-L protein was performed with immunohistostaining using an antibody recognizing human Fas-L. All nasopharyngeal tissues have a similar amount of transcripts of Fas-L. However, the Fas-L protein was detected exclusively on the cell surface of malignant epithelial cells of NPC. The present findings suggest that Fas-L protein may be involved in evading immune attack of NPC.

Original languageEnglish
Pages (from-to)421-424
Number of pages4
JournalOral Oncology
Volume35
Issue number4
DOIs
Publication statusPublished - 1999 Jul 1

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Fas Ligand Protein
Biopsy
Neoplasms
Southeastern Asia
Nasopharynx
Reverse Transcription
Nasopharyngeal carcinoma
Immune System
Epithelial Cells
Apoptosis
Carcinoma
Polymerase Chain Reaction
Antibodies
Incidence

All Science Journal Classification (ASJC) codes

  • Oncology

Cite this

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abstract = "Nasopharyngeal carcinoma (NPC) is an epithelial cancer with a high incidence in Southeast Asia. How it escapes attack from the host immune system is not fully understood. Recently, pieces of evidence show that Fas- ligand (Fas-L)-mediated apoptosis may be involved in immune privilege of tumours. To determine whether a similar mechanism may exist in NPC, the expression of Fas-L was analysed. Biopsy specimens of the nasopharynx were taken from 27 NPC patients. Histologically, they were either non-keratinizing or undifferentiated carcinomas. Nasopharyngeal biopsies of 11 other patients that proved to have no tumour served as control. The transcripts of Fas-L were detected by reverse transcription-polymerase chain reaction. Localization of Fas-L protein was performed with immunohistostaining using an antibody recognizing human Fas-L. All nasopharyngeal tissues have a similar amount of transcripts of Fas-L. However, the Fas-L protein was detected exclusively on the cell surface of malignant epithelial cells of NPC. The present findings suggest that Fas-L protein may be involved in evading immune attack of NPC.",
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Analysis of the expression of Fas-L in nasopharyngeal carcinoma tissues. / Tsai, Sen-Tien; Fang, Sheen-Yie; Jin, Yin Tai; Su, Ih Jen; Yang, Bei-Chang.

In: Oral Oncology, Vol. 35, No. 4, 01.07.1999, p. 421-424.

Research output: Contribution to journalArticle

TY - JOUR

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