Abstract
Nasopharyngeal carcinoma (NPC) is an epithelial cancer with a high incidence in Southeast Asia. How it escapes attack from the host immune system is not fully understood. Recently, pieces of evidence show that Fas- ligand (Fas-L)-mediated apoptosis may be involved in immune privilege of tumours. To determine whether a similar mechanism may exist in NPC, the expression of Fas-L was analysed. Biopsy specimens of the nasopharynx were taken from 27 NPC patients. Histologically, they were either non-keratinizing or undifferentiated carcinomas. Nasopharyngeal biopsies of 11 other patients that proved to have no tumour served as control. The transcripts of Fas-L were detected by reverse transcription-polymerase chain reaction. Localization of Fas-L protein was performed with immunohistostaining using an antibody recognizing human Fas-L. All nasopharyngeal tissues have a similar amount of transcripts of Fas-L. However, the Fas-L protein was detected exclusively on the cell surface of malignant epithelial cells of NPC. The present findings suggest that Fas-L protein may be involved in evading immune attack of NPC.
| Original language | English |
|---|---|
| Pages (from-to) | 421-424 |
| Number of pages | 4 |
| Journal | Oral Oncology |
| Volume | 35 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1999 Jul 1 |
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All Science Journal Classification (ASJC) codes
- Oncology
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Analysis of the expression of Fas-L in nasopharyngeal carcinoma tissues. / Tsai, Sen-Tien; Fang, Sheen-Yie; Jin, Yin Tai; Su, Ih Jen; Yang, Bei-Chang.
In: Oral Oncology, Vol. 35, No. 4, 01.07.1999, p. 421-424.Research output: Contribution to journal › Article
TY - JOUR
T1 - Analysis of the expression of Fas-L in nasopharyngeal carcinoma tissues
AU - Tsai, Sen-Tien
AU - Fang, Sheen-Yie
AU - Jin, Yin Tai
AU - Su, Ih Jen
AU - Yang, Bei-Chang
PY - 1999/7/1
Y1 - 1999/7/1
N2 - Nasopharyngeal carcinoma (NPC) is an epithelial cancer with a high incidence in Southeast Asia. How it escapes attack from the host immune system is not fully understood. Recently, pieces of evidence show that Fas- ligand (Fas-L)-mediated apoptosis may be involved in immune privilege of tumours. To determine whether a similar mechanism may exist in NPC, the expression of Fas-L was analysed. Biopsy specimens of the nasopharynx were taken from 27 NPC patients. Histologically, they were either non-keratinizing or undifferentiated carcinomas. Nasopharyngeal biopsies of 11 other patients that proved to have no tumour served as control. The transcripts of Fas-L were detected by reverse transcription-polymerase chain reaction. Localization of Fas-L protein was performed with immunohistostaining using an antibody recognizing human Fas-L. All nasopharyngeal tissues have a similar amount of transcripts of Fas-L. However, the Fas-L protein was detected exclusively on the cell surface of malignant epithelial cells of NPC. The present findings suggest that Fas-L protein may be involved in evading immune attack of NPC.
AB - Nasopharyngeal carcinoma (NPC) is an epithelial cancer with a high incidence in Southeast Asia. How it escapes attack from the host immune system is not fully understood. Recently, pieces of evidence show that Fas- ligand (Fas-L)-mediated apoptosis may be involved in immune privilege of tumours. To determine whether a similar mechanism may exist in NPC, the expression of Fas-L was analysed. Biopsy specimens of the nasopharynx were taken from 27 NPC patients. Histologically, they were either non-keratinizing or undifferentiated carcinomas. Nasopharyngeal biopsies of 11 other patients that proved to have no tumour served as control. The transcripts of Fas-L were detected by reverse transcription-polymerase chain reaction. Localization of Fas-L protein was performed with immunohistostaining using an antibody recognizing human Fas-L. All nasopharyngeal tissues have a similar amount of transcripts of Fas-L. However, the Fas-L protein was detected exclusively on the cell surface of malignant epithelial cells of NPC. The present findings suggest that Fas-L protein may be involved in evading immune attack of NPC.
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U2 - 10.1016/S1368-8375(99)00016-0
DO - 10.1016/S1368-8375(99)00016-0
M3 - Article
VL - 35
SP - 421
EP - 424
JO - Oral Oncology
JF - Oral Oncology
SN - 1368-8375
IS - 4
ER -