Analysis of the interaction between Zinc finger protein 179 (Znf179) and promyelocytic leukemia zinc finger (Plzf)

Ding-Yen Lin, Chi Chen Huang, Ya Ting Hsieh, Hsin Chuan Lin, Ping Chieh Pao, Jen Hui Tsou, Chien Ying Lai, Liang-Yi Hung, Ju-Ming Wang, Wen Chang Chang, Yi Chao Lee

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Zinc finger protein 179 (Znf179), also known as ring finger protein 112 (Rnf112), is a member of the RING finger protein family and plays an important role in neuronal differentiation. To investigate novel mechanisms of Znf179 regulation and function, we performed a yeast two-hybrid screen to identify Znf179-interacting proteins. Results: Using a yeast two-hybrid screen, we have identified promyelocytic leukemia zinc finger (Plzf) as a specific interacting protein of Znf179. Further analysis showed that the region containing the first two zinc fingers of Plzf is critical for its interaction with Znf179. Although the transcriptional regulatory activity of Plzf was not affected by Znf179 in the Gal4-dependent transcription assay system, the cellular localization of Znf179 was changed from cytoplasm to nucleus when Plzf was co-expressed. We also found that Znf179 interacted with Plzf and regulated Plzf protein expression. Conclusions: Our results showed that Znf179 interacted with Plzf, resulting in its translocation from cytoplasm to the nucleus and increase of Plzf protein abundance. Although the precise nature and role of the Znf179-Plzf interaction remain to be elucidated, both of these two genes are involved in the regulation of neurogenesis. Our finding provides further research direction for studying the molecular functions of Znf179.

Original languageEnglish
Article number98
JournalJournal of biomedical science
Volume20
Issue number1
DOIs
Publication statusPublished - 2013 Dec 20

Fingerprint

Zinc Fingers
Zinc
Proteins
Leukemia
Promyelocytic Leukemia Protein
Yeast
Cytoplasm
Yeasts

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

Cite this

Lin, Ding-Yen ; Huang, Chi Chen ; Hsieh, Ya Ting ; Lin, Hsin Chuan ; Pao, Ping Chieh ; Tsou, Jen Hui ; Lai, Chien Ying ; Hung, Liang-Yi ; Wang, Ju-Ming ; Chang, Wen Chang ; Lee, Yi Chao. / Analysis of the interaction between Zinc finger protein 179 (Znf179) and promyelocytic leukemia zinc finger (Plzf). In: Journal of biomedical science. 2013 ; Vol. 20, No. 1.
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title = "Analysis of the interaction between Zinc finger protein 179 (Znf179) and promyelocytic leukemia zinc finger (Plzf)",
abstract = "Background: Zinc finger protein 179 (Znf179), also known as ring finger protein 112 (Rnf112), is a member of the RING finger protein family and plays an important role in neuronal differentiation. To investigate novel mechanisms of Znf179 regulation and function, we performed a yeast two-hybrid screen to identify Znf179-interacting proteins. Results: Using a yeast two-hybrid screen, we have identified promyelocytic leukemia zinc finger (Plzf) as a specific interacting protein of Znf179. Further analysis showed that the region containing the first two zinc fingers of Plzf is critical for its interaction with Znf179. Although the transcriptional regulatory activity of Plzf was not affected by Znf179 in the Gal4-dependent transcription assay system, the cellular localization of Znf179 was changed from cytoplasm to nucleus when Plzf was co-expressed. We also found that Znf179 interacted with Plzf and regulated Plzf protein expression. Conclusions: Our results showed that Znf179 interacted with Plzf, resulting in its translocation from cytoplasm to the nucleus and increase of Plzf protein abundance. Although the precise nature and role of the Znf179-Plzf interaction remain to be elucidated, both of these two genes are involved in the regulation of neurogenesis. Our finding provides further research direction for studying the molecular functions of Znf179.",
author = "Ding-Yen Lin and Huang, {Chi Chen} and Hsieh, {Ya Ting} and Lin, {Hsin Chuan} and Pao, {Ping Chieh} and Tsou, {Jen Hui} and Lai, {Chien Ying} and Liang-Yi Hung and Ju-Ming Wang and Chang, {Wen Chang} and Lee, {Yi Chao}",
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Analysis of the interaction between Zinc finger protein 179 (Znf179) and promyelocytic leukemia zinc finger (Plzf). / Lin, Ding-Yen; Huang, Chi Chen; Hsieh, Ya Ting; Lin, Hsin Chuan; Pao, Ping Chieh; Tsou, Jen Hui; Lai, Chien Ying; Hung, Liang-Yi; Wang, Ju-Ming; Chang, Wen Chang; Lee, Yi Chao.

In: Journal of biomedical science, Vol. 20, No. 1, 98, 20.12.2013.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Analysis of the interaction between Zinc finger protein 179 (Znf179) and promyelocytic leukemia zinc finger (Plzf)

AU - Lin, Ding-Yen

AU - Huang, Chi Chen

AU - Hsieh, Ya Ting

AU - Lin, Hsin Chuan

AU - Pao, Ping Chieh

AU - Tsou, Jen Hui

AU - Lai, Chien Ying

AU - Hung, Liang-Yi

AU - Wang, Ju-Ming

AU - Chang, Wen Chang

AU - Lee, Yi Chao

PY - 2013/12/20

Y1 - 2013/12/20

N2 - Background: Zinc finger protein 179 (Znf179), also known as ring finger protein 112 (Rnf112), is a member of the RING finger protein family and plays an important role in neuronal differentiation. To investigate novel mechanisms of Znf179 regulation and function, we performed a yeast two-hybrid screen to identify Znf179-interacting proteins. Results: Using a yeast two-hybrid screen, we have identified promyelocytic leukemia zinc finger (Plzf) as a specific interacting protein of Znf179. Further analysis showed that the region containing the first two zinc fingers of Plzf is critical for its interaction with Znf179. Although the transcriptional regulatory activity of Plzf was not affected by Znf179 in the Gal4-dependent transcription assay system, the cellular localization of Znf179 was changed from cytoplasm to nucleus when Plzf was co-expressed. We also found that Znf179 interacted with Plzf and regulated Plzf protein expression. Conclusions: Our results showed that Znf179 interacted with Plzf, resulting in its translocation from cytoplasm to the nucleus and increase of Plzf protein abundance. Although the precise nature and role of the Znf179-Plzf interaction remain to be elucidated, both of these two genes are involved in the regulation of neurogenesis. Our finding provides further research direction for studying the molecular functions of Znf179.

AB - Background: Zinc finger protein 179 (Znf179), also known as ring finger protein 112 (Rnf112), is a member of the RING finger protein family and plays an important role in neuronal differentiation. To investigate novel mechanisms of Znf179 regulation and function, we performed a yeast two-hybrid screen to identify Znf179-interacting proteins. Results: Using a yeast two-hybrid screen, we have identified promyelocytic leukemia zinc finger (Plzf) as a specific interacting protein of Znf179. Further analysis showed that the region containing the first two zinc fingers of Plzf is critical for its interaction with Znf179. Although the transcriptional regulatory activity of Plzf was not affected by Znf179 in the Gal4-dependent transcription assay system, the cellular localization of Znf179 was changed from cytoplasm to nucleus when Plzf was co-expressed. We also found that Znf179 interacted with Plzf and regulated Plzf protein expression. Conclusions: Our results showed that Znf179 interacted with Plzf, resulting in its translocation from cytoplasm to the nucleus and increase of Plzf protein abundance. Although the precise nature and role of the Znf179-Plzf interaction remain to be elucidated, both of these two genes are involved in the regulation of neurogenesis. Our finding provides further research direction for studying the molecular functions of Znf179.

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SN - 1021-7770

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